Project description:Comparative trascriptomic analysis between porcine early-blastocyst and Hatched blastocyst collected around day 5-6 direct comparison with dye-swap; two different arrays with 6 Samples each
Project description:Regulatory Mechanisms of Atrial Remodeling of Mitral Regurgitation Pigs This study enrolled 6 pigs (age: 18 months) and divided into three groups: mitral regurgitation pigs (MR) (n = 2; 2 males sacrificed 12 months after surgery), MR pigs treated with valsartan (MRV) (n = 2; 2 males age-matched to MR sacrificed 12 months after surgery), and normal control pigs (NC) (n = 2; 2 males age-matched to MR pigs). Valsartan (3.43 mg/kg/day), a type I angiotensin II receptor blocker, was administered from one week before surgery and then daily after surgery in the MRV group. We sought to systemically elucidate critical differences in the alteration of RNA expression pattern between the atrial myocardium of pigs with and without MR, and between the atrial myocardium of MR pigs with and without valsartan using high-density oligonucleotide microarrays and functional network enrichment analysis.
Project description:Gene expression of characteristic chondrogenic markers and miRNA expression were analyzed in cells cultured in differentiation medium and significant differences were found between gelation/PRP microgels and those containing only pure gelatin. We used microarrays to detail the miRNA expression in studied cell cultures for identification the expression of miRNA and study the up- and down-regulated miRNA associated.
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Early embryonic development is highly sensitive to both developmental stage and culture environment, and in vitro fertilization (IVF) is known to induce transcriptional and epigenetic alterations that may compromise embryo quality and developmental competence. To systematically characterize stage-specific and origin-dependent transcriptional differences, we performed RNA sequencing of porcine embryos derived in vivo and by IVF at key preimplantation stages. Transcriptomes were generated from in vivo–derived blastocysts and hatched blastocysts, IVF-derived blastocysts and hatched blastocysts, as well as IVF-derived 4-cell embryos. Comparative analyses enable the dissection of transcriptional programs associated with early cleavage, blastocyst formation, hatching, and culture-induced perturbations. This dataset provides a comprehensive resource for investigating developmental stage transitions, IVF-associated transcriptional dysregulation, and molecular determinants of embryo quality during porcine preimplantation development.