Project description:Transcriptional profiling of A549, U2OS, and Nalm6 comparing dexamethason with vehicle control, 3 hour timepoint Two conditions (treated and untreated), three cell types. Biological replicates: 3 treated and 3 vehicle controls for each cell type. Each array is a two-color hyb of treated vs control in a single cell type (9 arrays total). One dye swap for each condition
Project description:To identify genome-wide CCCTC binding factor (CTCF)-binding in A549 (ATCC CCL-185) and U2OS cells stably expressing rat GR (Rogatsky et al. , Mol Cell Biol, 1997. 17(6): p. 3181-93.) we performed ChIP-seq experiments upon hormone treatment (1.5 h, 1 M dexamethasone).
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.