Project description:While our previous evolutionary analyses of Salinispora and its BGCs have focused on 118 strains isolated from worldwide marine sediment samples, the aim of this study was to isolate, genome-sequence, and characterize the biosynthetic potential of a "microscale" collection of Salinispora strains isolated from a 1-m^2 quadrat in Fiji, thus allowing us to compare taxonomic and BGC diversity at different spatial scales. This MassIVE data set contains LCMS runs of the ethyl acetate extracts produced from liquid cultures of 99 microscale Salinispora strains (96 are classified as S. arenicola, 2 as S. pacifica, and 1 as S. oceanensis) grown in A1 media, along with two media controls. The extracts were analyzed on a Agilent 1260 Infinity Series LC system coupled to a 6530 Series Q-TOF mass spectrometer, using a C18 Phenomenex Luna column (5 um, 100 mm x 4.6 mm) with a 10 min solvent gradient from 10% to 100% MeCN (0.1% FA) in water (0.1% FA) with 1.0 mL min-1 flow rate. LCMS parameters: positive mode, source ionization 0 eV, CID 30 eV and a 100-1700 Da detection window. Raw .d and converted .mzxml file formats are included.
