Project description:Skeletal elements from the feather star Anneissia japonica were isolated and stripped of organic material. The skeleton was then demineralized and the occluded proteins isolated. The proteins were separated by SDS-PAGE and fractions were analyzed by LC-MS/MS. The results were compared to predicted proteins encoded by the genome. The proteins found in the organic matrix of the skeleton were compared to those found in other skeletons both within Echinodermata and to other taxa.
Project description:Conserved polyadenylation by poly(A) polymerases (PAPs) is essential for mRNA stability, yet its metabolic functions remain unclear. Here we identify a rare variant p.D469N (Asp469Asn) in Star-PAP present in lean individuals but absent in obese cases. Adipose-specific Star-PAP knockout mice develop progressive adipose tissue loss, hepatic steatosis, and severe insulin resistance, phenocopying human lipodystrophy. Mechanistically, Star-PAP directly binds the the insulin receptor (InsR) mRNA 3’–UTR to stabilize its transcript. Both deletion of Star-PAP and p.D469N variant decrease InsR mRNA maturation. Star-PAP deficiency impaires insulin receptor signaling and adipocyte lipid storage, while restoring InsR in adipocytes partially ameliorates the lipodystrophic phenotypes. Notably, Star-PAP deficiency disruptes acetyl-CoA homeostasis secondary to insulin signaling defects, accompanied by reduced histone acetylation at fatty acid synthesis genes. Pyruvate supplementation effectively restores acetyl-CoA levels and histone acetylation, thereby rescuing the gene expression and lipogenesis. Together, our results identify Star-PAP-mediated RNA polyadenylation as a mechanism governing adipose homeostasis, positioning Star-PAP as a key regulator of insulin receptor signaling and lipodystrophy.
2026-09-08 | GSE345555 | GEO
Project description:Genome sequencing of Amblyomma americanum (lone star tick) adult principal pseudohaplotype
Project description:We employed HITS-CLIP to map genome wide Star-PAP mRNA binding and define the role of RBM10 on global Star-PAP mRNA association. We show a transcriptome-wide association of Star-PAP which is diminished on cellular Star-PAP depletion. HITs-CLIP data analysis of RBM10 knockdown and pulldown with anti Star-PAP antibody on HEK293 cells indicates significance of RBM10 in Star-PAP and mRNA association.
Project description:Strain ∆staRPZnstaR was obtained to investigate genome-wide differential gene expresion when staR gene is reppressed or overexpressed by adding Zn to the culture medium. In addition, gene expression profile alterations produced upon inhibition of gyrase with Novobiocin were studied in the absence and presence of StaR.
Project description:Sea stars achieve strong yet reversible underwater adhesion using a duo-gland system in their tube feet. Here we collected secreted footprint material from Asterina gibbosa tubefeet to identify sea star footprint proteins (Sfps).
Project description:Conserved polyadenylation by poly(A) polymerases (PAPs) is essential for mRNA stability, yet its metabolic functions remain unclear. Here we identify a rare variant p.D469N (Asp469Asn) in Star-PAP present in lean individuals but absent in obese cases. Adipose-specific Star-PAP knockout mice develop progressive adipose tissue loss, hepatic steatosis, and severe insulin resistance, phenocopying human lipodystrophy. Mechanistically, Star-PAP directly binds the the insulin receptor (InsR) mRNA 3’–UTR to stabilize its transcript. Both deletion of Star-PAP and p.D469N variant decrease InsR mRNA maturation. Star-PAP deficiency impaires insulin receptor signaling and adipocyte lipid storage, while restoring InsR in adipocytes partially ameliorates the lipodystrophic phenotypes. Notably, Star-PAP deficiency disruptes acetyl-CoA homeostasis secondary to insulin signaling defects, accompanied by reduced histone acetylation at fatty acid synthesis genes. Pyruvate supplementation effectively restores acetyl-CoA levels and histone acetylation, thereby rescuing the gene expression and lipogenesis. Together, our results identify Star-PAP-mediated RNA polyadenylation as a mechanism governing adipose homeostasis, positioning Star-PAP as a key regulator of insulin receptor signaling and lipodystrophy.