Project description:Arabidopsis ult1-3 mutant was shown to rescue the phenotype of LFYasEMF1 to that of WT. We performed microarray analysis of 7-d-old and 15-d-old seedlings of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 grown under short day to study the underlie mechanism of this phenotypic rescuing.
Project description:Arabidopsis ult1-3 mutant was shown to rescue the phenotype of LFYasEMF1 to that of WT. We performed microarray analysis of 7-d-old and 15-d-old seedlings of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 grown under short day to study the underlie mechanism of this phenotypic rescuing. Gene expression of 7 and 15 day old WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 seedlings grown under short day condition were performed and compared.
Project description:ULT1 and CLF function antagonistically as epigenetic regulators of gene expression in Arabidopsis. We sought to identity their global downstream target genes at two stages of plant development and determine their common targets. We used microarrays to determine the global gene expression programs regulated by ULT1 and FLC during vegetative and reproductive development and compared the overlap among target genes between the two proteins.
Project description:The early flowering Arabidopsis cäö mutant is defective in the BRR2a helicase involved in splicing. In order to get some explanation about the phenotype, we compared the expression profiles of wt and mutant with RNA-seq. In addtion, the same RNA-seq data was used to measure the extent of intron retention in the cäö mutant
Project description:SHORT-ROOT Deficiency Alleviates the Cell Death Phenotype of the Arabidopsis catalase2 Mutant under Photorespiration-Promoting Conditions
Project description:The oxt6 mutant is an oxidative stress-tolerant Arabidopsis mutant that is deficient in a polyadenylation factor subunit. Expression analysis suggests that impaired poly(A) site choice is responsible for the stress-tolerant phenotype. We used microarrays to understand the link between the polyadenylation defect and stress tolerance. Keywords: mutant, wild-type, complemented line comparison
