Project description:The CAMTA1 mutant and Col-0 were studied under water and drought condition. The camta1 showed stunted primary root growth under osmotic stress. The expression analysis revealed drought recovery as major indicative pathway along with membrane and chloroplast related protein in camta1 under drought stress. Large number of positively regulated genes were related to osmotic balance, transporters, AP2 and ABA. We used Affymetrix expression analysis to validate the role of CAMTA1 under drought stress. The RNA of Col-0 and camta1 mutant were isolated in water and drought condition.The ATH1 gene chip was used for expression analysis.
Project description:The CAMTA1 mutant and Col-0 were studied under water and drought condition. The camta1 showed stunted primary root growth under osmotic stress. The expression analysis revealed drought recovery as major indicative pathway along with membrane and chloroplast related protein in camta1 under drought stress. Large number of positively regulated genes were related to osmotic balance, transporters, AP2 and ABA. We used Affymetrix expression analysis to validate the role of CAMTA1 under drought stress.
Project description:Crop plants are often exposed to the combination of drought and pathogen stress. Transcriptome studies on Arabidopsis thaliana and other plants unveiled activation of shared molecular defense mechanisms between under individual and combined stresses. These shared plant responses are characterized by commonly regulated genes under individual and combined stresses. Based on the previous studies, G-box binding factor 3 (GBF3) is one of the regulatory components of such shared responses. However, the mechanistic understanding on the role of GBF3 under combined drought and pathogen stress is not yet decoded. Using genetic approaches, we demonstrated Atgbf3 mutant plants are more susceptible under individual and combined drought and Pseudomonas syringae pv. tomato DC3000 stresses as compared to the wild-type plants. We further analyzed the global transcriptome of Atgbf3 mutant under combined stress to identify its downstream targets to further validate the role of AtGBF3 in combined stress. We used microarrays to detail the global transcriptome reprogramming during AtGBF3-mediated regulation of combined stress.
Project description:MiRNAs are important plant regulators responsible for growth, development and stress responses in plants.Previous studies have shown the roles of these miRNA on individual stress conditions, however their role in combined stress conditions such as nitrogen deficiency and drought are still unclear. Understanding the molecular mechanisms underlying plant responses to combined drought and nitrogen deficiency stress is essential for developing multiple stress-tolerant crops. In this study, Arabidopsis thaliana calli were subjected to combined drought and nitrogen deficiency, RNA extracted and then high throughput sequencing analysis. Analysis identified several differentially expressed miRNAs upregulated and downregulated. We also uncovered the target genes of these miRNAs. Gene ontology analysis provided insights into the different molecular, biological and cellular processes of these target genes. Overall, our findings contribute to the understanding of miRNA-mediated regulatory mechanisms under combined plant stress responses and offer valuable insights for the development of stress-tolerant crop varieties in stressors.