Project description:The goal of this project is to compare the primary metabolite profile in different tissue types of the model plant Arabidopsis thaliana. Specifically, plants were grown hydroponically under the long-day (16hr light/day) condition at 21C. Tissue samples, including leaves, inflorescences, and roots were harvest 4 1/2 weeks post sowing. Untargeted primary metabolites profiling was carried out using GCTOF.
Project description:Elevated CO2 leads to major changes in plant physiology, including stimulation of growth and alteration of mineral content. In order to identify root genes that might be involved in the CO2 plant response, we grew Arabidopsis thaliana plants in hydroponics under contrasted levels of atmospheric CO2, nitrate and iron provision. Roots were collected and RNA-seq were performed.
Project description:The project intended to reveal protein phosphorylation patterns in Arabidopsis thaliana in response to ATP. For this purpose, Arabidopsis thaliana plants, including WT, ATP receptor mutants (p2k1, p2k2, and double mutant p2k1/p2k2), and P2K1 overexpression plants, were treated with ATP or buffer (as the negative control). Crude membrane proteins were then extracted, reduced with DTT, alkylated with iodoacetamide, and digested with Lys-C/trypsin. The digested peptides were then acidified with formic acid, desalted with C18 SPE columns, and concentrated in a Speed-Vac concentrator. The Phosphopeptides were enriched from the above digested peptide samples using IMAC and then analyzed with LC-MS/MS. Data was searched with MaxQuant (ver. 2.0.1.0), which identified and quantified peptides and proteins across all of with Arabidopsis thaliana data set (Uniprot.2020.11.02).
Project description:In this study we explain the physiological, biochemical and gene expression mechanisms adopted by ammonium nitrate-fed Arabidopsis thaliana plants growing under elevated [CO2], highlighting the importance of root-to-shoot interactions in these responses A transcriptomic analysis enabled the identification of photoassimilate allocation and remobilization as fundamental process used by the plants to maintain the outstanding photosynthetic performance. Moreover, based on the relationship between plant carbon status and hormone functioning, the transcriptomic analyses provided an explanation of why phenology accelerates in elevated [CO2] conditions.
