Project description:The aims of our study were: 1) to elucidate physiological responses in three Miscanthus species and newly bred triploid hybrid in three water stress treatment conditions 2) utilise the induced physiological conditions for an in-depth transcriptome study on the molecular basis of water stress in Miscanthus spp.
Project description:Cymbopogon spp. are grass species widely used around the world as medicinal plants and in various industries for the production of perfumes and pharmaceuticals. Despite their extensive use, there are relatively few studies at the genomic and transcriptomic levels. In this study, transcriptomic data were generated for two species—Cymbopogon flexuosus and Cymbopogon winterianus—to investigate evolutionary aspects and the expression of genes associated with secondary metabolite biosynthesis. The transcriptome assembly revealed a total of 18,286 and 22,458 transcripts for C. flexuosus and C. winterianus, respectively. Furthermore, the reads were mapped against the genomes of related species, including Andropogon gerardii, Sorghum bicolor, Saccharum officinarum, Miscanthus sinensis, Miscanthus lutarioriparius, and Zea mays, to assess their conservation across these genomes. The results indicated which species are more closely related to the Cymbopogon genus and highlighted key differences in metabolic pathways.
Project description:To dissect the temporal architecture of chilling resilience, we conducted an integrative, time-resolved analysis of two Miscanthus sinensis genotypes contrasting in chilling tolerance, Ms12 and Ms16. Through stepwise chilling and recovery treatments, we profiled genotype-specific changes in shoot physiology, hormone accumulation, gene expression, and importantly, cell wall composition.