Project description:MucR is one of the few transcriptional regulatory proteins that has been linked to Brucella pathogenesis. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to copare the gene expression properties of wild type and isogenic mucR mutant cells.
Project description:MucR is one of the few transcriptional regulatory proteins that has been linked to Brucella pathogenesis. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to copare the gene expression properties of wild type and isogenic mucR mutant cells. B. abortus strain 2308 or mucR mutant cells were grown to stationary phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of MucR.
Project description:MavR is a 160 nucleotide regulatory RNA molecule that is produced during B. abortus strain 2308 growth in nutrient-replete broth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic mavR mutant cells.
Project description:VtlR (virulence-associated transcriptional LysR-family regulator) is thought to be a transcriptional regulator of B. abortus virulence determinants We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic vtlR mutant cells.
Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)
Project description:VtlR (virulence-associated transcriptional LysR-family regulator) is thought to be a transcriptional regulator of B. abortus virulence determinants We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic vtlR mutant cells. B. abortus strain 2308 or vltR mutant cells were grown to late exponential phase growth, total bacterial RNA was isolated and subjected to GeneChip hybridization and analysis. We sought to determine the regulatory effects of VtlR.
Project description:abcR1 and abcR2 produce two regulatory RNA molecules, that are produced during stationary phase growth. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic abcR1 abcR2 double mutant cells.
Project description:The ybeY locus is hypothesized to produce an endoribonuclease, that is expressed during late exponential phase growth and regulate transcript titers. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic ybeY mutant cells.
