Project description:To elucidate the effect of heat stress and the following recovery on grapevines and identify some regulated genes representing the classical heat stress response and thermotolerance mechanisms, transcript abundance of grapevine (Vitis vinifera L.) were quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts), followed by quantitive Real-Time PCR validation for some transcript profiles. The treatment: heat stress(5h) and the following recovery (18.5h), sampling were conducted at two time respectively. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Lijun Wang. The equivalent experiment is VV40 at PLEXdb.]
Project description:Experimental research on the effects of abiotic stress over grapevine has mainly focused on water shortage. The adaptation of plants to stress is a complex response triggered by cascades of molecular networks involved in stress perception, signal transduction, and the expression of specific stress-related genes and metabolites. Approaches such as array-based transcript profiling allow assessing the expression of thousands of genes in control and stress tissues. Gene expression upon acute (heat and light) and steady (drought) individual stresses and field conditions were compared in two grapevine (Vitis vinifera L.) varieties, Trincadeira (TR) and Touriga Nacional (TN).
Project description:In order to investigate the putative roles of the VvPLCP genes in grapevine resistance, the leaves-specific expression patterns of VvPLCPs were analyzed according to transcriptome data in two cultivars including V. vinifera cv. ‘Zitian Seedless’ and Vitis rootstocks ‘Kober 5BB’ when infected with P. viticola
Project description:Study of gene expression during Plasmopara viticola infection in the resistant Vitis vinifera cultivar 'Regent'. The oomycete fungus Plasmopara viticola (Berk. et Curt.) Berl. et de Toni is responsible for grapevine downy mildew disease. Most of the cultivated grapevines are sensitive to this pathogen, thus requiring intensive fungicide treatments. The molecular basis of resistance to this pathogen is poorly understood. We have carried out a cDNA microarray transcriptome analysis to identify grapevine genes associated with resistance traits. Early transcriptional changes associated with downy mildew infection in the resistant Vitis vinifera cultivar ‘Regent’, when compared to the susceptible cultivar ‘Trincadeira’, were analyzed. Transcript levels were measured at three time-points: 0, 6 and 12 hours post inoculation (hpi). Our data indicate that resistance in V. vinifera ‘Regent’ is induced after infection. This study provides the identification of several candidate genes that may be related to ‘Regent’ defense mechanisms, allowing a better understanding of this cultivar's resistance traits.
Project description:Downy mildew, caused by the obligate biotrophic oomycete Plasmopara viticola, is one of the most destructive grapevine diseases worldwide, constituting a major challenge to viticulture. Because an increasing number of pesticides are removed from market due to their impact on human health and/or the environment, there is an increasing need for alternative strategies to control fungal diseases. Silica nanoparticles (SiO2 NPs) and Frangula anlus extract (Fa) are emerging as promising tools for sustainable plant disease management. While their ability to enhance disease resistance has been demonstrated in several crop species, their potential in grapevine (Vitis vinifera) remains poorly investigated. In this study, foliar application of SiO2 NPs and Fa significantly reduced P. viticola infection in grapevine under both controlled and field conditions. Among multiple assays to characerize their effects, transcriptomic response of SiO2 NP-treated and Fa-Treated, infected and non-infected leaves were evaluated and compared to transcriptomic response of acibenzolar-S-methyl (B) treatment, a well known plant-defence activator. Overall, these findings provide new insights into SiO2 NP-induced and Fa-induced responses in grapevine and highlight their potential for sustainable disease management.
Project description:Vitis vinifera endogenous small RNAs Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves, inflorescences, tendrils and small berries were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.
Project description:Night heat stress triggered different transcriptomic responses than day stress in green and ripening grapevine (Vitis vinifera) fruits
Project description:White grape (Vitis vinifera cv. Furmint) berry samples subjected to natural noble rot were collected in a vineyard in Mád, Hungary (Tokaj wine region). Raw data include grapevine and Botrytis cinerea sequence reads.
Project description:Size fractionated small RNA from total RNA extracts of Vitis vinifera leaves were ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Illumina high throughput pyrosequencing. The kit used is TrueSeq Small RNA kit Please see www.illumina.com for details of the sequencing technology.
