Project description:Plasma from pregnant women were applied three days in three steps to HUVEC cells. RNAs were isolated and prepared. Four pools of RNAs were obtained, two of them from cells exposed to preeclamptic plasma (2 and 3, corresponding to mild and severe preeclampsia, respectively) and two from cells exposed to normal plasma from pregnant women (five and five).
Project description:Plasma from normal and preeclamptic pregnancies was collected and added in the culture medium of HUVEC cells. The aims are double: identifying putative biomarkers that could be modified recurrently in the endothelium under preeclampsia and understand at term the molecular bases of the increased risk of cardiovascular and kidney diseases in woman that have had a preeclampsia. 2 pools of 3 control plasmas and 2 pools of 4 preeclamptic plasma were organized and added at a concentration of 1/10 on HUVEC cells, during 24 hours before transcriptomic analysis
Project description:Plasma from normal and preeclamptic pregnancies was collected and added in the culture medium of HUVEC cells. The aims are double: identifying putative biomarkers that could be modified recurrently in the endothelium under preeclampsia and understand at term the molecular bases of the increased risk of cardiovascular and kidney diseases in woman that have had a preeclampsia.
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex
Project description:Placental Tissue Samples from 36 women (17 normotensive women, denoted with a P, and 19 preeclamptic women, denoted with a Q) were analyzed for differenital methylation Preeclamptic womene were compared direclty to normotensive women controlling for gestational age, race, maternal age, and baby sex
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Analysis of ex vivo isolated lymphatic endothelial cells from the dermis of patients to define type 2 diabetes-induced changes. Results preveal aberrant dermal lymphangiogenesis and provide insight into its role in the pathogenesis of persistent skin inflammation in type 2 diabetes. The ex vivo dLEC transcriptome reveals a dramatic influence of the T2D environment on multiple molecular and cellular processes, mirroring the phenotypic changes seen in T2D affected skin. The positively and negatively correlated dLEC transcripts directly cohere to prolonged inflammatory periods and reduced infectious resistance of patients´ skin. Further, lymphatic vessels might be involved in tissue remodeling processes during T2D induced skin alterations associated with impaired wound healing and altered dermal architecture. Hence, dermal lymphatic vessels might be directly associated with T2D disease promotion. Global gene expression profile of normal dermal lymphatic endothelial cells (ndLECs) compared to dermal lymphatic endothelial cells derived from type 2 diabetic patients (dLECs).Quadruplicate biological samples were analyzed from human lymphatic endothelial cells (4 x diabetic; 4 x non-diabetic). subsets: 1 disease state set (dLECs), 1 control set (ndLECs)
