Bos taurus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA178260 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR609183.fastq.gz | Fastqsanger.gz | |||
| SRR609184.fastq.gz | Fastqsanger.gz | |||
| SRR609185.fastq.gz | Fastqsanger.gz | |||
| SRR609186.fastq.gz | Fastqsanger.gz | |||
| SRR609187.fastq.gz | Fastqsanger.gz |
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Project description:Digital dermatitis is a painful foot disease compromising welfare in dairy cattle. The disease has a complex multibacterial aetiology, but little is known about its pathogenesis. In this study, gene expression in skin biopsies from five bovine digital dermatitis lesions and five healthy bovine feet was compared using RNA-Seq technology. Differential gene expression was determined after mapping transcripts to the Btau 4.0 genome. Pathway analysis identified gene networks involving differentially expressed transcripts. Bovine digital dermatitis lesions had increased expression of mRNA for a2-macroglobulin-like 1, a protein potentially involved in bacterial immune evasion and bacterial survival. There was increased expression of keratin 6A and interleukin 1b mRNA in bovine digital dermatitis lesions, but reduced expression of most other keratin and keratin-associated genes. There was little evidence of local immune reactions to the bacterial infection present in lesions. Ten samples were processed; five normal skin biopsies from the hind foot skin and five digital dermatitis lesions (large (>2cm) red raw in appearance).
2013-03-26 | E-GEOD-41732 | biostudies-arrayexpress
Project description:Digital dermatitis is a painful foot disease compromising welfare in dairy cattle. The disease has a complex multibacterial aetiology, but little is known about its pathogenesis. In this study, gene expression in skin biopsies from five bovine digital dermatitis lesions and five healthy bovine feet was compared using RNA-Seq technology. Differential gene expression was determined after mapping transcripts to the Btau 4.0 genome. Pathway analysis identified gene networks involving differentially expressed transcripts. Bovine digital dermatitis lesions had increased expression of mRNA for a2-macroglobulin-like 1, a protein potentially involved in bacterial immune evasion and bacterial survival. There was increased expression of keratin 6A and interleukin 1b mRNA in bovine digital dermatitis lesions, but reduced expression of most other keratin and keratin-associated genes. There was little evidence of local immune reactions to the bacterial infection present in lesions.
2013-03-26 | GSE41732 | GEO
Project description:Extracellular vesicles (EVs) are key mediators of intercellular communication, and often play critical roles in host-parasite interactions by facilitating parasite’s physiology and pathogenesis. Theileria annulata, an apicomplexan parasite, induces profound changes in host cells, leading to uncontrolled proliferation, apoptosis resistance, and increased invasiveness. In this study, we performed the comprehensive proteomic and small RNA analysis of EVs isolated from a T. annulata Kashi isolate-infected bovine lymphocyte cell line (TaXJS), B cell line (TaBC), dendritic cell line (TaDC), and from the sera of cattle before and after infection. Our label-free LC-MS/MS proteomics identified 2580 proteins, while small RNA sequencing revealed 6635 miRNAs associated with parasite development, host invasion, and immune evasion. Functional enrichment analyses recognized vesicular components involved in key pathways of the parasite-host such as ECM-receptor interaction, oxidative phosphorylation, and proton transport. These findings highlight the potential of Theileria-derived EVs in modulating host responses and their potential as therapeutic and vaccine targets.
2025-09-29 | PXD056906 | Pride
Project description:ABSTRACT Digital dermatitis (DD) is a painful inflammatory skin disease affecting the digital regions of cattle feet, linked to pathogenic Treponemes spp. This condition imposes a substantial economic burden on the cattle industry attributable to lameness and subsequent production inefficiencies. Currently, there is an absence of fully effective therapeutics for DD, and the underlying mechanisms governing the skin's inflammatory response remain largely unknown. This study elucidated the proteomic signature in the foot skin of cattle manifesting active DD lesions subjected to topical treatment with a non-antimicrobial metalloprotease inhibitor, CMC2.24 or the routinely used antibiotic treatment, oxytetracycline (OTC). The results demonstrated that active, non-healing ulcerative DD lesions exhibited persistent infiltration of inflammatory leukocytes alongside a proteomic profile characterized by enrichment in neutrophil degranulation-related pathways and elevated activity of matrix metalloproteinases (MMPs), which likely exacerbates cutaneous inflammation and ulceration. Treatment with OTC accelerated the cornification of DD lesions; however, these lesions still exhibited distinct inflammatory characteristics, which included sustained elevated levels of S100A8, a molecule known to promote neutrophil aggregation and reactive oxygen species (ROS) generation. Conversely, the MMP inhibitor CMC2.24 effectively mitigated active DD lesions by attenuating ROS production and keratinization pathways. Furthermore, CMC2.24 inhibited interleukin-1 beta (Il-1β) secretion in both bovine and murine macrophages challenged with DD-associated Treponemas spp. These data indicate a pro-inflammatory proteomic profile in DD lesions, highlighting that a non-antimicrobial MMP inhibitor could effectively reduce foot skin inflammation relative to conventional OTC treatment.
2025-09-19 | PXD060742 | Pride
Project description:Targeting the treponemal microbiome of digital dermatitis infections by deep coverage pyrosequencing
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Project description:We examined 36 biopsies taken from digital dermatitis lesions of Holstein cows. The target was the V3 -V4 variable region of 16S rRNA using Treponema specific primers. We identified 20 different taxa of Treponema using this approach. Phylogenetic study of the Treponema taxa found in digital dermatitis lesions of Holstein cows.
2013-06-25 | E-GEOD-42426 | biostudies-arrayexpress
Project description:Here, we report the first complete genomes of three cultivable treponeme species from bovine digital dermatitis (DD) skin lesions, two comparative human treponemes and a bovine gastrointestinal (GI) isolate. Key genomic differences between bovine and human treponemes implicate microbial mechanisms that enhance knowledge of how DD, a severe disease of ruminants, has emerged into a prolific and endemic disease in countries worldwide. Bovine DD treponemes have additional oxidative stress genes compared to nearest human-isolated relatives, suggesting better toleration of oxidative stress, and thus potentially explaining the ability of bovine strains to colonize skin surfaces. Comparison of bovine DD and GI treponemes as well as bovine pathogenic and human non-pathogenic saprophyte Treponema phagedenis strains indicates genes encoding a five-enzyme biosynthetic pathway for the production of 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid, a rare di-N-acetylated mannuronic acid sugar with potential immunomodulatory activity, as important for pathogenesis. The bovine T. phagedenis strains further differed from human strains by having a larger number of unique genetic features including components of a type IV secretion system and a phosphate utilisation system including phoU, a gene associated with enhanced osmotic stress survival. Proteomic analyses confirmed bovine derived T. phagedenis exhibits expression of PhoU but not the putative secretion system whilst the novel mannuronic acid pathway was expressed in near entirety across the DD treponemes. Analysis of osmotic stress response in water identified a difference between bovine and human T. phagedenis with bovine strains surviving better. This novel mechanism could enable a selective advantage, allowing environmental persistence and transmission of bovine T. phagedenis. Finally, we investigated a range of treponeme genes encoding ortholog families across the DD treponemes representing putative outer membrane proteins (OMPs) and identified several families as multi-specific adhesins capable of binding extra cellular matrix (ECM) components. Human saprophytic T. phagedenis could not be differentiated from bovine pathogenic strains by these adhesins and only two of ten characterised ortholog OMP families were absent from commensal bovine treponemes. One of these bovine pathogen specific adhesin ortholog families showed considerable diagnostic potential with the Treponema medium representative demonstrating the greatest disease specificity (91.6%). This work has shed light on treponeme host adaptation and has identified candidate molecules for future diagnostics, vaccination and therapeutic intervention.
2021-03-16 | PXD022419 | Pride
Project description:Skin lesions from Digital Dermatitis Metagenome
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