Rattus norvegicus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA179264 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Role of p300 in regulation of stress-response genes in heart is not clear. Treatment with anti-cancer drug Doxorubicin causes cardiotoxicity through generation of oxidative stress in cardiac myocytes. Loss of p300 enhances cell death and apoptosis in response to Doxorubicin. The objective of this study was to determine if expression of genes regulated by oxidative stress is dependent of presence of p300. Further we investigated if protective effect of p300 against Doxorubicin was due to gene expression effects of p300. We used microarray to compare the transcriptomes of cardiac myocytes transfected with anti-p300 or non-silencing siRNA beforebefore and after exposure to Doxorubicin.
2015-10-01 | GSE42177 | GEO
Project description:Biological sex influences gene expressions in cardiac myocytes
| PRJNA376550 | ENA
Project description:Expression data from individual MEF2A isoform knockdown in neonatal rat ventricular myocytes (NRVMs)
| PRJNA358666 | ENA
Project description:Novel targets of microRNA miR-142 in cardiac myocytes
| PRJNA144955 | ENA
Project description:Expression data from pooled biological replicates (n=6) from MEF2D knockdown in neonatal rat ventricular myocytes (NRVMs)
| PRJNA293215 | ENA
Project description:Knee osteoarthritis (KOA), as a degenerative multifactorial disease, affects the quality of life and mental health of patients, and also brings a huge socioeconomic burden. Treating synovitis have shown promise as anti-inflammatory therapeutics in mitigating OA symptoms and disease progression. Here, by analysing synovial single-cell sequencing (scRNA-seq) data from KOA, we found that synovial fibroblasts (FLS) in OA synovium showed a distinct pro-inflammatory phenotype. We collected synovial tissue from patients with clinical OA as well as from healthy donors, and histological examination was consistent with findings in scRNA-seq. Inspired by recent cross-tissue fibroblast lineage studies, we identified by sequencing that healthy FLS in synovial tissues share transcriptome-level similarities with dermal fibroblasts (DFb). Subsequently, we revealed the local as well as systemic distribution of intra-articular injected DFbs by constructing/extracting two types of rat fibroblasts (luciferase DFbs as well as GFP DFbs). The results demonstrate that DFbs can be locally retained in the synovium for up to three weeks following targeted engrafting on it. And intra-articular injection does not result in DFbs migration to vital organs or the occurrence of histological changes in these organs. A rat model of KOA was constructed by anterior cruciate ligament transection (ACLT) in order to study the therapeutic effect of DFbs on KOA. After injection, the rats showed improvement in painful gait. In addition, histological as well as imaging results showed reduced synovitis and improvement in articular cartilage. Finally we verified the protective effect of DFbs on cytokine-stimulated chondrocytes in a co-culture system.
2026-06-24 | GSE304724 | GEO
Project description:Expression data from cardiac stem cells (CSCs) with or without MSC-exosomes treatment
| PRJNA297407 | ENA
Project description:Transcriptomic profiling of neonatal rat ventricular myocytes under LPS stimulation with ESRRG overexpression or knockdown
| PRJNA1291615 | ENA
Project description:miRNA expression data from neonatal rat ventricular myocytes (NRVM) silenced for KChIP2
| PRJNA305405 | ENA
Project description:Expression data from isolated perfused rat hearts exposed to doxorubicin
| PRJNA113641 | ENA