Project description:This SuperSeries is composed of the following subset Series: GSE42323: Transcriptional Responses to Sleep in Peripheral Tissues (Heart) GSE42324: Transcriptional Responses to Sleep in Peripheral Tissues (Lung) Refer to individual Series
Project description:While central circuits governing sleep are well-studied, the contribution of signaling from peripheral tissues remains a critical yet less understood aspect of sleep regulation. The highly conserved RNA-binding protein Pumilio (Pum) is a post-transcriptional regulator expressed in multiple tissues that influence systemic physiology, but its role in modulating basal sleep has not been established. Although Pumilio’s function in central neurons has been linked to sleep homeostasis following deprivation, whether it regulates sleep through peripheral mechanisms remains unknown. Here, we use conditional genetic tools in the fruit fly Drosophila melanogaster to demonstrate that genetic manipulation of Pumilio targeting the intestinal stem cells (ISCs) and the endocrine corpus allatum (CA) regulates the transition to sleep. Reducing Pumilio function in either the ISCs or the CA independently and significantly accelerates nighttime sleep onset, while overexpression produces the opposite effect. This behavioral change is accompanied by widespread transcriptional alterations in the head, characterized by a robust upregulation of genes involved in cellular stress responses. Our findings reveal a previously unrecognized gut-endocrine-brain signaling axis and identify peripheral post-transcriptional regulation as a key input to the central control of sleep behavior.
Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (count table) only. Raw data will be submitted to EGA.
Project description:Molecular profiles in sleep and sleep deprivation in peripheral tissues using microarrays Time point study. Mice were sacrificed by cervical dislocaton following 3, 6, 9, and 12 h of total sleep deprivation (n = 8 or 9 at each time point). Deprivation was initiated at lights-on and performed through gentle handling.
Project description:Molecular profiles in sleep and sleep deprivation in peripheral tissues using microarrays Time point study. Mice were sacrificed by cervical dislocaton following 3, 6, 9, and 12 h of total sleep deprivation (n = 8 or 9 at each time point). Deprivation was initiated at lights-on and performed through gentle handling.
Project description:We investigated the genomic and physiological impact of acute sleep loss in peripheral tissues, by obtaining adipose tissue and skeletal muscle after one night of sleep loss and after one full night of sleep. Processed data (M-values for probes not overlapping SNPs) only. Raw data will be submitted to EGA.
