Project description:Lobosphaera incisa is an oleaginous green microalga and a target organism of high biotechnological interest because it is the richest known plant source of arachidonic acid (ARA), a pharmaceutically and nutraceutically valuable omega-6 long-chain polyunsaturated fatty acid. ARA is deposited in considerable amounts in storage lipid triacylglycerols, most profoundly when L. incisa is exposed to nitrogen starvation. To get insights into genome-wide gene expression in L. incisa under nitrogen starvation, the whole transcriptome was sequenced by RNA-Seq. Total RNA was isolated under four growth conditions: exponential growth (control) and growth in nitrogen-depleted medium for 12 or 72 hours at normal or high light intensities. Statistical and differential gene expression analyses were conducted by comparing the various samples. The results of this study were used to identify patterns of gene regulation with special emphasis on genes involved in fatty acid and lipid metabolism.
Project description:Prunus subgenus Ceresus is a renowned ornamental wooden plant for its landscape applications and economic value in gardens. However, waterlogging during the rainy season in some areas cause deaths and heavy losses. Fortunately, we have found that a variety of Prunus subgenus Ceresus × Incisa 'Gotenba zakura' could generate aerial roots when suffering from heavy rains and sustainable floods. In this study, we conducted an associated analysis to explore the core regulating network of aerial root growth mechanism in Prunus subgenus Ceresus × Incisa 'Gotenba zakura' by combining phenological observations, physiological assays, and transcriptome comparisons across five separate stages. By analyzing the heatmap of DEGs and gene co-expression network (GCN), we have recognized genes that may play very critical roles under waterlogging stress. Gene network indicates that aerial roots can improve the tolerance under waterlogging by ROS degradation, endogenous hormone induction, as well as energy production. This discover provides a valid foundation of waterlogging tolerance of Prunus subgenus Ceresus, and molecular evidence for selecting promising rootstocks.