Project description:Transcriptional profiling of human acute lymphoblastic leukemia cell lines RS4;11: compare untreated RS4;11 with RS4;11 treated with VE-465. The goal was to determine the effects of VE-465 on global gene expression in RS4;11.
Project description:Transcriptional profiling of human acute lymphoblastic leukemia cell lines RS4;11: compare untreated RS4;11 with RS4;11 treated with VE-465. The goal was to determine the effects of VE-465 on global gene expression in RS4;11. VE-465 induced gene expression in RS4;11 was measured at 48 hours after exposure to doses of 0 and 200 nM VE-465. Three independent experiments were performed.
Project description:We generated recombinant human sVE-cadherin (extracellular domains EC1-5) which was applied to human dermal microvascular endothelial cells (HDMEC) to investigate it`s role in endothelial barrier function. In other experiments we observed that sVE-cadherin disrupted endothelial barrier function by dismantling the VE-cadherin complex at cell borders via VE-PTP-dependent RhoA activation. The sequencing project compares untreated HDMEC vs treated cells.
