Project description:We performed single-cell RNA-sequencing to create a cell census of the human thymus during development, early childhood and adult life. We sampled 15 embryonic and fetal thymi spanning thymic developmental stages between 7 post-conception weeks (PCW) to 17 PCW, and 9 postnatal thymi from paediatric and adult samples. We compared the cellular composition and T cell differentiation within human and mouse thymus using newly generated and repository mouse datasets. Finally, we investigated the bias in the recombination and selection of human versus mouse TCR repertoires.
Project description:we mapped the locations of DNA segments occupied by GATA1 using chromatin immunoprecipitation (ChIP). We have produced genome-wide GATA1 ChIP datasets after restoration and activation in G1E-ER4 cells. we employed the sequence census methodology of ChIP-seq , using Illumina GA2 technology to produce 23 million reads (36 nucleotides long) uniquely mapped to the mouse genome (mm8 assembly) for the GATA1 ChIP DNA and 15 million mapped reads for the input DNA Examination of transcription factor GATA1 occupancy
Project description:In this study, we have carried out a broad census of histone PTMs in Ectocarpus chromatin and have developed a method to evaluate the genome-wide distribution of specific marks. We show that modulation of the expression of sporophyte-biased genes during the life cycle is correlated with marked changes in the pattern of three histone PTMs. In contrast, the expression patterns of gametophyte-biased genes were not correlated with modifications to the histone PTMs assayed, suggesting that gametophyte-biased and sporophyte-biased gene expression are mediated by different epigenetic processes.