Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA184735 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Ehf is a transcriptional regulator that is highly expressed and enriched in corneal epithelium. To gain insights into the role of Ehf in the corneal epithelium, we performed siRNA knockdown of Ehf in primary human corneal epithelial cells. Primary human corneal epithelial cells were transfected with siEhf or si controls, plated, and harvested at 72 hr.
2013-01-01 | E-GEOD-43157 | biostudies-arrayexpress
Project description:Ehf is a transcriptional regulator that is highly expressed and enriched in corneal epithelium. To gain insights into the role of Ehf in the corneal epithelium, we performed siRNA knockdown of Ehf in primary human corneal epithelial cells.
2013-01-01 | GSE43157 | GEO
Project description:The cornea, composed of epithelium, stroma and endothelium, protects the anterior compartment of the eye from damage and allows transmission of light into the eye. While well described morphologically, no studies have investigated the global gene expression changes in the cornea throughout the mouseM-bM-^@M-^Ys life. We characterized the global gene expression profile of mouse cornea from early development through aging, and compared to gene expression in other epithelial tissue, to identify cornea enriched genes, pathways, and transcriptional regulators. We identified Ehf, an ets family transcription factor, as being highly selectively expressed in the corneal epithelium compared to the stroma, and highly expressed in cornea compared to other epithelial tissues. siRNA experiments and Ehf ChIP-Seq on mouse corneal epithelium confirm the role of this factor in promoting epithelial identity and cell differentiation, and suggest it carries out these functions through interactions with other cornea epithelial differentiation factors including Klf4. Whole eye globes were dissected from wild type CB6 mice. Corneal epithelium was isolated by digestion in 50% EMEM/dispase II with 50 mM sorbitol for two hours at 37M-BM-0C. ChIP was performed with an Ehf antibody, and was sequenced with an input control.
2013-08-27 | E-GEOD-44741 | biostudies-arrayexpress
Project description:Ets homologous factor (EHF) is an Ets family transcription factor expressed in many epithelial cell types including those lining the respiratory system. Disruption of the airway epithelium is central to many lung diseases, and a network of transcription factors coordinates its normal function. EHF can act as a transcriptional activator or a repressor, though its targets in lung epithelial cells are largely uncharacterized. RNA-seq after EHF depletion or overexpression showed significant alterations in the expression of genes involved in response to wounding. EHF knockdown also targeted genes in pathways of epithelial development and differentiation and locomotory behavior. mRNA profiles from Calu-3 cells treated with negative control (NC) or EHF siRNA, in quintuplicate. mRNA profiles from 3 pcDNA (empty vector control) clones and 3 pcDNA-EHF overexpression A549 clones, 3-4 replicates each.
2014-12-23 | E-GEOD-63397 | biostudies-arrayexpress
Project description:The cornea, composed of epithelium, stroma and endothelium, protects the anterior compartment of the eye from damage and allows transmission of light into the eye. While well described morphologically, no studies have investigated the global gene expression changes in the cornea throughout the mouse’s life. We characterized the global gene expression profile of mouse cornea from early development through aging, and compared to gene expression in other epithelial tissue, to identify cornea enriched genes, pathways, and transcriptional regulators. We identified Ehf, an ets family transcription factor, as being highly selectively expressed in the corneal epithelium compared to the stroma, and highly expressed in cornea compared to other epithelial tissues. siRNA experiments and Ehf ChIP-Seq on mouse corneal epithelium confirm the role of this factor in promoting epithelial identity and cell differentiation, and suggest it carries out these functions through interactions with other cornea epithelial differentiation factors including Klf4.
2013-08-27 | GSE44741 | GEO
Project description:Expression data from human iPSC-derived corneal epithelial cells
| PRJNA298629 | ENA
Project description:PURPOSE. Building on identification of ABCB5 as a limbal stem cell (LSC) marker, this study evaluates CD63, a newly identified LSC molecule co-expressed with ABCB5, in human limbal epithelial cells to elucidate its role in maintaining corneal epithelial identity. METHODS. RNA sequencing (RNA-seq) was performed on sorted Abcb5-positive and Abcb5-negative murine corneal epithelial cells. CD63 expression in human corneal tissue was assessed by flow cytometry and immunostaining. siRNA-mediated knockdown (KD) of CD63 was conducted in cultured human limbal epithelial cells. Subsequent analyses included qRT-PCR, flow cytometry, RNA-seq, and western blotting. RESULTS. RNA-seq analysis of mouse corneal epithelial cells revealed high expression of LSC markers, including Krt15, Krt6b, Fgfr1, Gpha2, Ifitm3, and Cd63, as well as low expression of differentiation-associated markers, such as Krt12 and Gja1, in Abcb5-positive cells. In human corneal tissue, flow cytometry and immunostaining confirmed robust CD63 expression in the limbal region. siRNA-mediated KD of CD63 in cultured human limbal epithelial cells resulted in a marked reduction in the expression of corneal epithelium-enriched genes, including KRT12, CLU, ALDH1A1, ALDH3A1, TGFBI and MYEOV. Notably, CD63 KD resulted in a ~50 % decrease in expression of a key transcriptional regulator of corneal epithelial identity, PAX6. CONCLUSIONS. CD63 is abundantly expressed in the human limbus and plays a critical role in maintaining the expression of corneal epithelium-specific proteins, likely through modulation of PAX6. These findings underscore the functional significance of CD63 in limbal stem cell biology and its essential contribution to corneal epithelial homeostasis.
2026-03-18 | GSE308189 | GEO
Project description:MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-016436 array data]
| PRJNA133461 | ENA
Project description:MicroRNA-145 Regulates Human Corneal Epithelial Differentiation [Agilent-014850 array data]
| PRJNA133463 | ENA
Project description:To understand the gene-regulatory role of H19 in corneal epithelial cells and to explore whether CLIM activation of H19 might account for non-cell proliferation effects of CLIM, we used siRNA to knock down H19 in primary human corneal epithelial cells. Microarray gene expression analysis revealed that 1,249 genes are down regulated 1.5 fold or more by siH19 compared to scramble control, and that 944 genes are up regulated
2016-04-28 | GSE80387 | GEO