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Arabidopsis thaliana

ABSTRACT: Involvement of microRNA-related regulatory pathways in glucose-mediated control of Arabidopsis early seedling development

PROVIDER: PRJNA186682 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR649537.fastq.gz	Fastqsanger.gz
	SRR649538.fastq.gz	Fastqsanger.gz
	SRR649539.fastq.gz	Fastqsanger.gz

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Involvement of microRNA-related regulatory pathways in glucose-mediated control of Arabidopsis early seedling development

Project description:In plants, sugars such as glucose act as signaling molecules that promote changes in gene expression programs impacting on growth and development. Recent evidences have revealed the potential participation of mRNA decay control in some aspects of glucose-mediated regulatory responses suggesting a role of microRNAs (miRNAs) in these responses. In order to get a better understanding of glucose-mediated development modulation involving miRNA-related regulatory pathways, early seedling development of mutants impaired in miRNA biogenesis (hyl1-2) and miRNA-activity (ago1-25) were evaluated. Both mutants exhibited a glucose hyposensitive phenotype from germination up to seedling establishment, indicating that miRNA regulatory pathways may be involved in the glucose-mediated delay of early seedling development. The expression profile of 200 primary miRNA transcripts (pri-miRs) were evaluated by large-scale quantitative real-time polymerase chain reaction (qRT-PCR) profiling revealing that 38 pri-miRs were regulated by glucose. For several of them, the corresponding mature miRNAs are known to participate directly or indirectly in plant development, and their accumulation was shown to be co-regulated with the pri-miRNA by glucose. These data indicate that deficiency in miRNA machinery leads to a deregulated expression of several miRNA target genes in response to glucose. Also, such deficiencies result in glucose-promoted misexpression of genes for the three Abscisic Acid signaling elements ABI3, ABI4 and ABI5. Thus, miRNA-regulatory pathways play a role in the adjustments of growth and development triggered by glucose signaling. Three samples, including untreated control, 2% glucose-treated and 2% mannitol-treated samples.

2013-09-06 | E-GEOD-43546 | biostudies-arrayexpress

Involvement of microRNA-related regulatory pathways in glucose-mediated control of Arabidopsis early seedling development

2013-09-06 | GSE43546 | GEO

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Project description:AtbZIP16 integrates light and hormone signaling pathways to regulate Arabidopsis early seedling development

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Project description:Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

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Project description:Arabidopsis seedling were exposed in co-culture to E. amylovora mVOC and data show that mVOCs promote plant growth and early responses

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Time-series of the seed-to-seedling transition in Arabidopsis thaliana

Project description:The expression levels of Arabidopsis thaliana (Col-0) genes in several developmental stages during the seed-to-seedling transition were measured by using high-density Affymetrix® arrays (Aragene.st1.1). We used a time-series of microarrays to gain temporal resolution and identify relevant genes in the seed-to-seedling transition.

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Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

2013-04-05 | GSE40245 | GEO

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Project description:Expression data from Arabidopsis seedling

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Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.

2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress

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