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Homo sapiens

ABSTRACT: Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling

PROVIDER: PRJNA186783 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR650353_1.fastq.gz	Fastqsanger.gz
	SRR650353_2.fastq.gz	Fastqsanger.gz
	SRR650354_1.fastq.gz	Fastqsanger.gz
	SRR650354_2.fastq.gz	Fastqsanger.gz
	SRR650355_1.fastq.gz	Fastqsanger.gz

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Evaluation of the effectiveness of semen collection and sperm purification methods for spermatozoa transcript profiling

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Project description:The objective of the present study is to investigate if females have the ability to recognise X or Y chromosome bearing spermatozoa and present a different response to different spermatozoa. In order to investigate if females have the ability to recognise X or Y chromosome bearing spermatozoa and present a different response to different spermatozoa. Samples of semen were sorted for chromosomal sex by M-oM-,M-^Bow cytometric-sorting. Using laparoscopic insemination one oviduct was inseminated with X spermatozoa and the contralateral oviduct was inseminated with Y spermatozoa.This allowed us to obtain samples from the oviduct in contact with X sperm and Y sperm in the the contrlateral oviduct from the same sow. Oviduct pig samples were collected for RNA isolation and hybridization on Affymetrix microarrays. Four biological replicates were performed (n= 4 sows) and a total of 8 arrays were used for microarrays study (4 arrays for oviduct samples in contact with X spermatozoa and 4 arrays for oviduct samples in contact with Y spermatozoa.

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