Project description:This study investigates transcriptomic responses of Pacific salmon lice, Lepeophtheirus salmonis, to infection with the microsporidian Facilispora margolisi and/or exposure to emamectin benzoate (EMB), an antiparasitic agent commonly used in salmon aquaculture.
Project description:LC-MS/MS data of metabolites produced by Streptomyces baldaccii NRRL B-3500 grown in monoculture (ISP2, A-medium, and RAM2) and grasshopper-associated conditions.
The dataset includes monoculture extracts, grasshopper-associated samples (+GH), grasshopper controls without bacteria (BLK+GH), and methanol extracts prepared with (YW) or without (NW) aqueous pretreatment.
Data were acquired using an Agilent 6550 iFunnel Q-TOF LC/MS system.
Project description:Intestinal microbiota shape host immunity, but whether they can pre-activate defense against intracellular pathogens remains unclear. Here, we identify a natural microbiome member, Stenotrophomonas indicatrix JUb19, as the first example of a bacterium that partially induces the Intracellular Pathogen Response in Caenorhabditis elegans in the absence of infection. This response is coupled to broader metabolic remodeling, suggesting that combined immune and metabolic changes contribute to defense. We show that bacterial single-stranded RNA contributes to this effect, identifying a novel microbiome-derived signal that links microbial exposure to host defense. JUb19 exposure reduces susceptibility to both viral and microsporidian pathogens and confers inherited protection to unexposed progeny, but at a cost to parental fitness. These findings reveal a mechanism by which microbiome-derived RNA programs immunity against intracellular pathogens across generations.
Project description:Microsporidia are intracellular eukaryotic pathogens that pose a substantial threat to immunocompromised hosts. The way these pathogens manipulate host cells during infection remains poorly understood. Using a proximity biotinylation strategy we established that microsporidian EnP1 is a nucleus-targeted effector that modifies the host cell environment. EnP1's translocation to the host nucleus is meditated by nuclear localization signals (NLSs). In the nucleus, EnP1 interacts with host histone H2B. This interaction disrupts H2B monoubiquitination (H2Bub), subsequently impacting p53 expression. Crucially, this inhibition of p53 weakens its control over the downstream target gene SLC7A11, enhancing the host cell's resilience against ferroptosis during microsporidian infection. This favorable condition promotes the proliferation of microsporidia within the host cell. These findings shed light on the molecular mechanisms by which microsporidia modify their host cells to facilitate their survival.
