Project description:HLB is suggested to be caused by the phloem-limited fastidious prokaryotic α-proteobacterium “Candidatus Liberibacter spp.” Previous studies focused on the proteome and transcriptome analyses of citrus 5 to 35-week-after “Ca. L. spp.” inoculation. In this study, gene expression profiles was analyzed using mandarin of Citrus reticulate Blanco cv. jiaogan leaves after 2-year infection with “Ca. L. asiaticus”. The Affymetrix GeneChip® citrus genome were applied to study the molecular pathways mediated by “Ca. L. asiaticus” inoculated 3-year-old jiaogan seedlings. Each of them was graft-inoculated with one sweet orange scions with or without “Ca. L. asiaticus” in Dectember, 2009. RNA samples from three mandarin trees infected with 'Candidatus Liberibacter asiaticus' and three uninfected trees were used for affymatrix genochip
Project description:Oedaleus asiaticus is a dominant grasshopper in northern China’s grasslands and agro-pastoral zones, yet its growth and development miRNA regulation remains poorly understood. Here, we performed small RNA sequencing on female O. asiaticus at three developmental stages: 3-day-old third-instar nymphs, 3-day-old fifth-instar nymphs, and newly emerged adults. We identified 365 miRNAs (291 known, 74 novel), with 75 differentially expressed across stages. Target prediction for 43 differentially expressed miRNAs yielded 42,773 putative targets.GO and KEGG enrichment analysis were performed on the target gene set to reveal the biological processes, cell components, molecular functions and metabolic signaling pathways involved in these target genes. This study presents the first miRNA expression profiling of O. asiaticus, providing a valuable resource for future growth and development regulatory mechanism research.
