Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA187986 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR652430.fastq.gz | Fastqsanger.gz | |||
| SRR652431.fastq.gz | Fastqsanger.gz | |||
| SRR652432.fastq.gz | Fastqsanger.gz | |||
| SRR652433.fastq.gz | Fastqsanger.gz | |||
| SRR652434.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 6 |
Similar Datasets
Project description:Transcriptome analysis in mouse late spermatocyte deficient in pachytene piRNAs (Miwi+/- and -/-)
| PRJNA178747 | ENA
Project description:Transcriptome analysis in mouse late spermatocyte deficient in pachytene piRNAs (MIWI-immunoprecipitated RNAs)
| PRJNA264072 | ENA
Project description:Transcriptome analysis in mouse early round spermatids deficient in pachytene piRNAs (Miwi+/- and -/-)
| PRJNA178743 | ENA
Project description:In animal germline cells, Piwi-interacting RNAs (piRNAs) silence retrotransposons through post-transcriptional and transcriptional mechanisms. However, little is known, especially in mammals, about the functions of piRNAs beyond retrotransposon suppression1-5. In mammalian spermatocytes, piRNAs are known to be abundantly expressed6-10. Here, we show that a subset of coding and noncoding RNAs in mouse spermatocytes is degraded by the piRNA pathway. By analyzing the germline trasnscriptome of mice deficient in piRNA biogenesis, we identify hundreds of mRNAs as direct targets of piRNAs. Remarkably, the 3' untranslated region (UTR) of the mRNAs up-regulated in the piRNA pathway mutants are highly enriched with retrotransposon sequenes, implying that these sequences serve as regulatory elements for piRNA-mediated regulation. Furthermore, deficiencies of piRNAs derived from pseudogenes result in increased mRNA levels of their cognate genes, indicating that pseudogenes regulate their functional cognates via piRNAs. Moreover, we identify a large population of testis-enriched long intergenic noncoding RNAs (lincRNAs), some of which are also degraded by the piRNA pathway. Collectively, our results reveal that the piRNA pathway regulates the expression of both mRNAs and lincRNAs in addition to retrotransposon RNAs during meiosis and the key role of retrotransposons and pseudogenes, two major types of genomic sequences, in this regulation by acting as piRNA sources and/or regulatory elements in target RNAs. Late spermatocyte mRNA profiles of Mov10l1 fl/+ and Mov10l1 s fl/- were analyzed by deep sequencing, in triplicate, using Illumina HiSeq.
2014-11-20 | E-GEOD-43735 | biostudies-arrayexpress
Project description:GC-2cell (a mouse pachytene spermatocyte-derived cell line) Genome sequencing and assembly
| PRJNA608077 | ENA
Project description:Mass spectrometry data of RNA interactome capture (RIC) assay for STA-PUT purified mouse primary male germ cells, including spermatogonia (SG), pachytene spermatocyte (pSC) and round spermatid (rST)
2025-10-29 | PXD043074 | Pride
Project description:We created mice, which are deficient for Myc specifically in cardiac myocytes by crossing crossed Myc-floxed mice (Mycfl/fl) and MLC-2VCre/+ mice. Serial analysis of earlier stages of gestation revealed that Myc-deficient mice died prematurely at E13.5-14.5. Morphological analyses of E13.5 Myc-null embryos showed normal ventricular size and structure; however, decreased cardiac myocyte proliferation and increased apoptosis was observed. BrdU incorporation rates were also decreased significantly in Myc-null myocardium. Myc-null mice displayed a 3.67-fold increase in apoptotic cardiomyocytes by TUNEL assay. We examined global gene expression using oligonucleotide microarrays. Numerous genes involved in mitochondrial death pathways were dysregulated including Bnip3L and Birc2. Keywords: wildtype vs Myc-null
2007-06-30 | GSE7162 | GEO
Project description:Pachytene piRNAs target endogenous mRNAs for silencing during meiosis
| PRJNA270239 | ENA
Project description:In animal germline cells, Piwi-interacting RNAs (piRNAs) silence retrotransposons through post-transcriptional and transcriptional mechanisms. However, little is known, especially in mammals, about the functions of piRNAs beyond retrotransposon suppression1-5. In mammalian spermatocytes, piRNAs are known to be abundantly expressed6-10. Here, we show that a subset of coding and noncoding RNAs in mouse spermatocytes is degraded by the piRNA pathway. By analyzing the germline trasnscriptome of mice deficient in piRNA biogenesis, we identify hundreds of mRNAs as direct targets of piRNAs. Remarkably, the 3' untranslated region (UTR) of the mRNAs up-regulated in the piRNA pathway mutants are highly enriched with retrotransposon sequenes, implying that these sequences serve as regulatory elements for piRNA-mediated regulation. Furthermore, deficiencies of piRNAs derived from pseudogenes result in increased mRNA levels of their cognate genes, indicating that pseudogenes regulate their functional cognates via piRNAs. Moreover, we identify a large population of testis-enriched long intergenic noncoding RNAs (lincRNAs), some of which are also degraded by the piRNA pathway. Collectively, our results reveal that the piRNA pathway regulates the expression of both mRNAs and lincRNAs in addition to retrotransposon RNAs during meiosis and the key role of retrotransposons and pseudogenes, two major types of genomic sequences, in this regulation by acting as piRNA sources and/or regulatory elements in target RNAs. mRNAs in leptotene/zygotene spermatocytes, early-pachytene spermatocytes, mid-pachytene spermatocytes, late pachytene/diplotene spermatocytes and round spermatids were analyzed by deep sequencing using Illumina HiSeq.
2014-11-20 | E-GEOD-41976 | biostudies-arrayexpress