Project description:To further investigate the potential functions of Streptomyces in the gut, the bile acid-induction experiment was conducted utilizing mass spectrometry for identification. Bile acids (BAs), crucial primary metabolites of cholesterol metabolism, play a vital role in lipid digestion and absorption, enterohepatic circulation, and metabolic regulation. Notably, elevated intestinal bile acid levels are observed during high-fat diet consumption. In the present study, Streptomyces microflavus was stimulated with four distinct bile acids: CA, DCA, CDCA, and UDCA. Subsequently, bacterial cells were harvested, proteins extracted, and data-independent acquisition (DIA)-based proteomic identification performed.
Project description:Investigation of whole genome gene expression level changes in Lactococcus lactis KCTC 3769T,L. raffinolactis DSM 20443T, L. plantarum DSM 20686T, L. fujiensis JSM 16395T, L. garvieae KCTC 3772T, L. piscium DSM 6634T and L. chungangensis CAU 28T . This proves that transcriptional profiling can facilitate in elucidating the genetic distance between closely related strains.
Project description:We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).
