Project description:Female domestic pigs were fed a 16-week Lean or Obese diet. Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in swine adipose-derived MSCs, and evaluated their reversibility using an epigenetic modulator, vitamin-C.
