Project description:Nakamurella lactea DLS-10T, isolated from rock in Korea, is one of the four type strains of the genus Nakamurella. In this study, we describe the high quality draft genome of N. lactea DLS-10T and its annotation. A summary of phenotypic data collected from previously published studies was also included. The genome of strain DLS-10T presents a size of 5.82 Mpb, 5100 protein coding genes, and a C + G content of 68.9%. Based on the genome analysis, emended description of N. lactea in terms of G + C content was also proposed.
Project description:White rot fungi, such as Irpex lacteus, offer significant potential for lignocellulose degradation and protein synthesis in sustainable biofuel and animal feed production,but optimizing nitrogen sources to balance fungal growth, lignin degradation, and protein synthesis remains a critical challenge.This study investigated the effects of ammonium chloride (NH₄Cl) and sodium nitrate (NaNO₃) on I. lacteus fermentation in wheat straw, with a focus on the regulation of nitrogen source.Transcriptomic analysis revealed that NaNO₃ upregulated genes associated with nitrogen uptake and cellulose/hemicellulose degradation,while exerting less repression on key ligninolytic enzymes, such as manganese peroxidase and cytochrome P450, compared to NH₄Cl.These findings suggest that NaNO₃ enhances I. lacteus metabolism by harmonizing lignin degradation with efficient nitrogen conversion.