Project description:To obtain genes expression in different parts of 84k poplar stems, transcriptome sequencing was performed using Illumina Novaseq 6000 second-generation sequencing platform from Shanghai BIOZERON Co. Ltd (www.biozeron.com). Selecte three stem segments of plants REPEAT 1, 2 and 3 with good and similar growth to use: 2nd-3rd internodes (poplar stem top: PST1, PST2, PST3); 9th-10th internodes (poplar stem middle: PSM1, PSM2, PSM3); 14th-15th internodes (poplar stem bottom: PSB1, PSB2, PSB3). [Or the three repeating organisms are also called poplar A, B, C. From top to bottom, the three parts of the stem are also called stem 1, 2, 3.]
Project description:Young poplar stems show a preponderantly primary growth in the top internodes and differential cambium activity in the basal internodes after inclination with some tension wood formed after 45 min. This study was conducted in order to characterize the early changes in gene expression during early stages of the gravitropic response in the poplar. Hybridizations were performed to compare gravistimulated poplar stems (basal region) after 45 min of inclination and untreated control stems. Two biological replicates were done. Two hybridizations with a dye swap for each tree pair were done, making a total of four hybridizations.
Project description:Young poplar stems show a preponderantly primary growth in the top internodes and differential cambium activity in the basal internodes after inclination with some tension wood formed after 45 min. This study was conducted in order to characterize the early changes in gene expression during early stages of the gravitropic response in the poplar.
Project description:Here we applied a novel approach to isolate nuclei from complex plant tissues (https://doi.org/10.1371/journal.pone.0251149), to dissect the transcriptome profiling of the hybrid poplar (Populus tremula × alba) vegetative shoot apex at single-cell resolution.
Project description:gravitropic stimulation / isotropic light-Characterization of early molecular events of woody stem gravitropism independently of phototropic responses
Project description:12plex_poplar_2012-01 - gravitropic stimulation / isotropic light - The experiments were designed to identify genes responding to the gravitropic stimulus in the xylem of poplar stem independently of phototropic responses. - young poplar trees were staked one week before use. For the experiment, the plants were placed in an isotropic light environment and were tited at 35° during 30 min. Control plants were kept straight in the isotropic light device. Xylem samples were collected from the upper and lower face of the stems. 4 biological replicates were made (4 controls and 4 tilted plants). RNA was extracted from each xylem sample. cDNA was synthetized. The discovery of genes whose expression is modulated by tilting was performed by a microarray approach.
Project description:Tropospheric ozone (O3) harms vegetation by reducing tree biomass and crop yield. Accurate risk assessment based on O3 uptake depends on quantifying the plant's capacity to cope with O3-generated reactive oxygen species (ROS) at the cellular level. Young leaves were shown to have better ability to deal with O3 stress than mature leaves but the mechanisms behind remain unclear. The aim of this study was to assess the crosstalk between O3 response and leaf development process. Time-course response to O3 (80 and 100 ppb) was studied at transcriptomic and cellular level in a growing leaf (GL) and an expanded leaf (EL) of young poplar. Quantification of hypersensitive response-like (HR-like) and chlorophyll showed that GL was more tolerant to O3 than EL. The response of leaf transcriptome to O3 concerns mostly genes regulated at the developmental level leading to an acceleration of leaf aging and senescence. GL response to O3 was delayed compared to EL suggesting that O3 tolerance at early stages of leaf development may result from an insensitivity to O3-induced senescence. In addition to detoxification mechanisms, understanding this process could offer new perspectives for O3 tolerance improvement or assessment.
