Project description:Sperm cryopreservation is widely used in zebrafish conservation, but its long-term impacts on offspring impacts remains unclear. This study aims to evaluate whether cryopreservation of zebrafish sperm affects the metabolism of adult offspring. Adult zebrafish generated from cryopreserved sperm are compared to those from fresh sperm. Liver samples are subjected to sequencingto assess changes in potential functional pathways. The results will provide insights into the long-term impact of gamete cryopreservation on offspring metabolism.
Project description:We analyzed if genomic responses of adult zebrafish tissues can reproduce the mammalian known inflammatory process induced by acute endotoxin stress. Although the strength of the inflammatory process was influenced by tissue nature, gene regulation was well conserved across evolution and zebrafish genomic responses highly correlated with mammals’ inflammatory reactions after lipopolysaccharide stimulation.
