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Opsanus beta strain:(Gulf Toadfish)

ABSTRACT: The sequence of Opsanus beta (Gulf Toadfish)

PROVIDER: PRJNA196921 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1662170_1.fastq.gz	Fastqsanger.gz
	SRR1662170_2.fastq.gz	Fastqsanger.gz
	SRR1661501_1.fastq.gz	Fastqsanger.gz
	SRR1661501_2.fastq.gz	Fastqsanger.gz
	SRR1662171_1.fastq.gz	Fastqsanger.gz

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Analysis of the Gulf toadfish intestinal proteome response to chronic hypersalinity exposure

Project description:The marine teleost intestine plays a vital role in whole body salt and water homeostasis. Marine fish must drink seawater in order to rehydrate, and processing of that seawater throughout the gastrointestinal tract allows for the extraction of water from this highly hyperosmotic source. Although the molecular mechanisms of this process have been the subject of much investigation, numerous questions remain. Here, Gulf toadfish (Opsanus beta) were acclimated to normal seawater (35 ppt) of hypersaline seawater (60 ppt) and changes in the anterior intestine, posterior intestine, and intestinal fluid proteomes were investigated using a shotgun proteomics approach employing isobaric TMT tags.

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Long-read de novo genome assembly of Gulf toadfish (Opsanus beta).

Project description:BackgroundThe family Batrachoididae are a group of ecologically important teleost fishes with unique life histories, behavior, and physiology that has made them popular model organisms. Batrachoididae remain understudied in the realm of genomics, with only four reference genome assemblies available for the family, with three being highly fragmented and not up to current assembly standards. Among these is the Gulf toadfish, Opsanus beta, a model organism for serotonin physiology which has recently been bred in captivity.ResultsHere we present a new, de novo genome and transcriptome assemblies for the Gulf toadfish using PacBio long read technology. The genome size of the final assembly is 2.1 gigabases, which is among the largest teleost genomes. This new assembly improves significantly upon the currently available reference for Opsanus beta with a final scaffold count of 62, of which 23 are chromosome scale, an N50 of 98,402,768, and a BUSCO completeness score of 97.3%. Annotation with ab initio and transcriptome-based methods generated 41,076 gene models. The genome is highly repetitive, with ~ 70% of the genome composed of simple repeats and transposable elements. Satellite DNA analysis identified potential telomeric and centromeric regions.ConclusionsThis improved assembly represents a valuable resource for future research using this important model organism and to teleost genomics more broadly.

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U.S. Gulf South

Project description:U.S. Gulf South Raw sequence reads

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Transcription profiling of blood from Gulf War Illness sufferers and control veterans before and after exercise

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