Project description:To investigate gene expression in different cells of Chara braunii we performed RNA sequencing of whole thali with rhizoids and thalli without rhizoids in strains NIES 1604 and S276.
Project description:Membrane fractions from Chara australis internodal cells were prepared by differential centrifugation. Chara cells were incubated in normal light/dark cycles or in the dark to obtain chaasome-enriched and depleted sample, respectively. Additionally, acidic and alkaline bands of individual Chara cells were separated for membrane preparations, too.