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Mucor circinelloides

ABSTRACT: Mucor circinelloides Small RNA sequencing

PROVIDER: PRJNA200295 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR1576768.fastq.gz	Fastqsanger.gz
	SRR835448.fastq.gz	Fastqsanger.gz
	SRR836081.fastq.gz	Fastqsanger.gz
	SRR836082.fastq.gz	Fastqsanger.gz

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ChIP-seq with Mucor circinelloides Mis12- and Dsn1-mCherry proteins

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Project description:We sequenced Endogenous short RNAs in Mucor circinelloides fungus grown in standard liquid culture. Short RNAs were profiled in wild type, Dicer-like 1 mutant (dcl1-), Dicer-like 2 mutant (dcl2-) and double Dicer mutant (dcl1-/dcl2-) strains. We identified many loci that produced less short RNAs in the dcl2- strain suggesting that DCL2 is the major protein generating short RNAs in Mucor circinelloides.

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Endogenous short RNAs in Mucor circinelloides sequenced on the Illumina platform (Solexa)

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Project description:Mucor circinelloides Genome sequencing

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Host-pathogen transcriptomics of Mucor circinelloides spores phagocytosed by mouse macrophages

Project description:Here, we have studied the first stage of this infection—the interaction of Mucor circinelloides spores with phagocytic cells—from an integrated transcriptomic and functional perspective. Our transcriptomic results showed that a relevant gene network is remodelled in response to phagocytosis, enriched in crucial functions to survive the phagosome, such as nutritional adaptation and response to oxidative stress. An additional transcriptomic analysis of atf1 and atf2 mutants unveiled the complex gene network of secondarily regulated genes involved in the response to phagocytosis. These new insights into the initial phase of mucormycosis define genetic regulators and molecular processes that could serve as pharmacological targets.

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