Project description:Transcription profiling by array of primary megakaryocytes (MEG) and erythroblasts (ERY) developed from murine fetal liver hematopoietic stem cells

2015-10-13 | E-GEOD-49664 | ExpressionAtlas

Mus musculus hematopoietic stem cell

2009-05-20 | GSE16139 | GEO

Mus musculus

Project description:Microarray data of mouse fetal liver

| PRJNA105965 | ENA

E-MTAB-8922

Project description:RNA from young and aged mouse hematopoietic stem cells

2020-04-03 | E-MTAB-8922 | ExpressionAtlas

Mus musculus hematopoietic stem cell

Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes. Mouse hematopoietic stem cells were purified from bone marrow cells using negative and positive selection with a Magnetic-Activated Cell Sorter (MACS). total RNA and mRNA were purified from the purified cells using Trizol reagent and magnetic oligo dT beads. Double strand cDNAs were synthesized using a cDNA synthesis kit and anchored oligo dT primers. After NlaIII digestion, 3’ cDNAs were isolated and amplified through 16-cycle PCR. SAGE tags were released from the 3’ cDNA after linker ligation. Ditags were formed, concatemerized and cloned into a pZERO vector. Sequencing reactions were performed with the ET sequencing terminator kit. Sequences were collected using a Megabase 1000 sequencer. SAGE tag sequences were extracted using SAGE 2000 software.

2009-05-20 | E-GEOD-16139 | biostudies-arrayexpress

E-GEOD-35961

Project description:Expression data from mouse liver treated with metformin

2015-09-28 | E-GEOD-35961 | ExpressionAtlas