Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Asthma is a chronic inflammatory airway disease characterized by airway inflammation and remodeling. The role of 15-oxo-5Z,8Z,11Z,13E-eicosatetraenoic acid (15-oxoETE), a 15-HETE metabolite catalyzed by 15-prostaglandin dehydrogenase (15-PGDH), has been relatively unexplored in asthma. In this study, we used RNA-seq to explore the effect of 15-KETE on the transcriptome of airway epithelial cells, aiming to identify its potential downstream targets and mechanisms of action.
Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type human coronavirus strain 229E (HCoV-229E)(ncbitaxon:11137) infection. Sample data was obtained for mock and infected (MOI 3) immortalized human lung epithelial cells (A549), immortalized human lung fibroblasts cells (MRC5), and primary human airway epithelial cells from lung tissue, and processed for proteome expression analysis using Limited Proteolysis (LiP) methods for Tandem Mass Tag 16-Plex (TMT16) and global proteomic measurements. Sample data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant software (v1.6.17.0).
Project description:The purpose of this experiment was to evaluate the human host cellular response to wild-type human coronavirus strain 229E (HCoV-229E)(ncbitaxon:11137) infection. Sample data was obtained for mock and infected (MOI 3) immortalized human lung epithelial cells (A549), immortalized human lung fibroblasts cells (MRC5), and primary human airway epithelial cells from lung tissue, and processed for proteome expression analysis using Limited Proteolysis (LiP) methods for Tandem Mass Tag 16-Plex (TMT16) and global proteomic measurements. Sample data was acquired using a Q-Exactive HF-X mass spectrometer and data was processed and compiled using MaxQuant software (v1.6.17.0).
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A6
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs. Two-condition experiment, KP MSCs vs. 3A6 MSCs.