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Mus musculus

ABSTRACT: The tetraspanin CD9 affords high purity capture of all murine hematopoietic stem cells.

PROVIDER: PRJNA207347 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR886461.fastq.gz	Fastqsanger.gz
	SRR886462.fastq.gz	Fastqsanger.gz
	SRR886463.fastq.gz	Fastqsanger.gz
	SRR886464.fastq.gz	Fastqsanger.gz

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The tetraspanin CD9 affords high purity capture of all murine hematopoietic stem cells.

Project description:Prospective isolation is critical to understand the cellular and molecular aspects of stem cell heterogeneity. Here we identify the cell surface antigen CD9 as a novel positive marker that provides a simple alternative for hematopoietic stem cell-isolation at high purity

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The tetraspanin CD9 affords high purity capture of all murine hematopoietic stem cells.

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Mechanical stress enhances CD9 expression in cultured podocytes

Project description:Transcriptomes of differentiated cells of the conditionally immortalized mouse podocyte cell line SVI (Schiwek et al., Kidney Int. 66: 91-101, 2004) were determined as described in Warsow et al. (Kidney Int. 84: 104-115, 2013) after application of mechanical stress (Endlich et al., J. Am. Soc. Nephrol. 12: 413-422, 2001) as compared to control conditions. Elevated glomerular pressure represents a high risk for the development of severe kidney diseases and causes an increase of mechanical load to podocytes. In this study we investigated whether mechanical stress alters gene expression in cultured podocytes using gene arrays. We found that tetraspanin CD9 is significantly upregulated in cultured podocytes after mechanical stress. The differential expression of CD9 was confirmed by RT-PCR and Western blot under stretched and unstretched conditions. Furthermore, mechanical stress resulted in a relocalization of CD9. To get an insight into the functional role of CD9, podocytes were transfected with pEGFP-CD9. The expression of CD9 induced the formation of substratum-attached thin arborized protrusions (TAPs). Ca2+ depletion revealed that podocytes over-expressing CD9 possess altered adhesive properties in contrast to the control transfected cells. Finally, elevated CD9 expression increased migration of podocytes in a wound assay. In summary, our results suggest that upregulation of CD9 may play an important role in podocyte morphology, adhesion and migration. Three independent batches were used.

2015-02-27 | E-GEOD-66336 | biostudies-arrayexpress

Microarray analysis of miRNA in breast cancer cell lines

Project description:The project aimed to identify miRNA that varied with tumourigenicity in human breast cell lines. The second objective was to identify miRNA that had differential expression between cell lines that varied in accordance with the abundance of the tetraspanin CD9 in the cell lines and the degree to which endogenous factors within these cell lines interacted with the 3'UTR of CD9 as determined by dual luciferase assay. These miRNA were considered potential regulators of the abundance of CD9 in breast cancer.

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Mechanical stress enhances CD9 expression in cultured podocytes

2015-02-27 | GSE66336 | GEO

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Project description:Transcriptome analysis of murine hematopoietic stem cells

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