Bos taurus
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ABSTRACT:
PROVIDER: PRJNA207549 | ENA |
REPOSITORIES: ENA
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Project description:Mycoplasma bovis is one of the major causative pathogens of the bovine respiratory complex disease that is characterized by enzootic pneumonia, mastitis, pleuritis and polyarthritis. M. bovis enters and colonizes the bovine respiratory epithelia through inhalation of aerosol from contaminated air. The nature of the interaction between M. bovis and bovine innate immune system is not well understood. We hypothesized that M. bovis invades blood monocytes and regulates cellular function to support its persistence and systemic dissemination. We used bovine-specific peptide kinome arrays to identify cellular signaling pathways that could be relevant to M. bovis-monocyte interaction in vitro. We validated these pathways using functional, protein and gene expression arrays. Here we show that infection of blood monocytes with M. bovis delays spontaneous or TNF-α/staurosporine-driven apoptosis, activates NF-κβ p65 subunit and inhibits caspase-9 activity. We also report that M. bovis infected bovine monocytes do not produce IFN-γ and TNF-α, although production of IL-10 is elevated. Our findings suggest that M. bovis takes over the cellular machinery of bovine monocytes to prolong bacterial survival and to possibly facilitate subsequent systemic distribution.
2016-06-07 | GSE47743 | GEO
Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine peripheral blood mononuclear cells play an important role for mycoplasma mastitis, however, the effects of M. bovis for immune response of peripheral blood mononuclear cells have not been fully clarified.We examined the transcription profiling of bovine peripheral blood mononuclear cells in intramammary infusion of M. bovis at day 7.
2019-12-19 | E-MTAB-7905 | biostudies-arrayexpress
Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine neutrophils play an important role for the eradication of pathogens which cause mycoplasmal infection, however the effects of M. bovis for immune response of neutrophils have not been fully clarified. We examined the transcription profiling of bovine neutrophils on the stimulation with M. bovis for 3h (3 stimuli, 3 control).
2020-11-30 | E-MTAB-9022 | biostudies-arrayexpress
Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine peripheral blood mononuclear cells (PBMCs) play an important role for the eradication of pathogens which cause mycoplasmal infection, however the effects of M. bovis for immune response of PBMCs in vitro have not been fully clarified.We examined the transcription profiling of bovine PBMCs on the stimulation with M. bovis for 6h (3 stimuli, 3 control).
2020-11-30 | E-MTAB-9023 | biostudies-arrayexpress
Project description:The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells and, endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, mammary gland tissue samples were collected from Holstein cows and screened for Mycoplasma bovis. Then, total RNA was isolated from mycoplasma bovis infected tissues and RNA sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. Our results revaled that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis case caused by Mycoplasma bovis.
2021-05-25 | GSE152865 | GEO
Project description:Mycoplasma bovis (M. bovis) causes to the diseases such as arthritis, pneumonia, abortion and mastitis causing to great losses in dairy bovine industries. It undertakes significant functions in the regulation of the immune response formed by many RNA type bacteria such as Messenger RNA (mRNAs) and Long noncoding RNA (lncRNAs). mRNA and lncRNA expression profiles still cannot be majorly known in the bovine mammary gland tissues infected with M. bovis. To illuminate this issue, transcription analysis has been conducted regarding mRNA and IncRNAs in the breast tissues belonging to Holstein cattle infected and not infected with M. Bovis. Differently expressed 1310 mRNAs and 57 IncRNAs have been determined in the bovine mammary gland tissues infected and not infected with M. Bovis. In addition; 392 novel lncRNAs have been detected. Also; it has been found that 19 novel lncRNAs are expressed in a different way. It has been revealed in gene ontology analysis that the differently expressed mRNAs and IncRNAs play a significant role in many vital biological pathways such as Metabolic pathways, T cell receptor signaling pathway, TGF-beta signaling pathway, Pathways in cancer, PI3K-Akt signaling pathway, NF-kappa B signaling pathway, mTOR signaling pathway and Apoptosis including the immune response and cancer. When literature review is conducted, it is seen that this study is the first genome wide IncRNA research conducted on the infected bovine mammary gland tissues with M. bovis agent. Our results have provided bovine mammary gland IncRNA and mRNA resource to understand their roles in the regulation of the immune response against the agent of M. Bovis in the bovine mammary gland tissues.
2020-06-01 | GSE133333 | GEO
Project description:Mycoplasma species are highly contagious pathogens, and intramammary Mycoplasma infection is a serious issue for the dairy industry. The bovine mammary epithelial cells (bMEC) play an important role for the eradication of pathogens which cause intramammary infection, however the effects of M. bovis for immune response of bMEC have not been fully clarified. We examined the transcription profiling of bMEC on the stimulation with M. bovis for 6h (3 stimuli, 3 control).
2017-11-30 | E-MTAB-5306 | biostudies-arrayexpress
Project description:Mycoplasma bovis mastitis is becoming increasingly problematic for dairy cattle farming in different parts of the world. M. bovis is inherently resistant to several antimicrobial classes and there is no effective vaccine. The major constraints to developing effective control tools are limited knowledge of M. bovis virulence factors and the underlying pathogenic mechanisms. The objective of this study was to determine virulence-associated genes of M. bovis and host immune response genes expressed during the early stages of host-pathogen interactions. In this study, we conducted in vitro infection of mammary epithelial cell (MAC-T) lines and in vivo intramammary infection of dairy cows with M. bovis strain PG45 and evaluated whole transcriptome differential gene expression and pathway enrichment analysis. We found a total of 614 (304 up-regulated, 310 down-regulated) and 7161 (3935 up-regulated, 3226 down-regulated) genes of M. bovis and bovine host cells were differentially expressed, respectively. Of the up-regulated genes of M. bovis, insertion sequence (IS) genes that are involved in transposase activity such as ISMbov1, ISMbov2, ISMbov3, and ISMbov9 were significantly up-regulated, whereas protein translation-associated genes were significantly down-regulated. In MAC-T cells, genes involved in apoptosis pathways and proinflammatory cytokines were significantly up-regulated, whereas genes involved in cell cycle, ribosome biogenesis, and steroid biosynthesis were significantly down-regulated. Genes encoding formation of neutrophil extracellular traps and proinflammatory cytokines, were significantly up-regulated in the mammary gland of M. bovis challenged cows, whereas genes involved in steroid biosynthesis and metabolism were significantly down-regulated. In conclusion, there were increased expressions of IS elements which are involved in transposase activity during early stages of M. bovis infection. This observation warrants further detailed investigation to determine important specific potential targets involved in the transposition for intervention. Apoptosis might be in favor of progression of M. bovis infection but intervention that reduces apoptosis and enhances quick and effective polymorphonuclear neutrophilic recruitment with increased extracellular trap activity may improve M. bovis clearance. Our findings are key to determining important targets for immunity-based intervention. To our knowledge, this is the first whole transcriptome analysis in both M. bovis and the host under the same in vitro and in vivo conditions. Therefore, we recommend additional metatranscriptomic, metaproteomic, metabolomic and metagenomic evaluation of M. bovis and other potentially non-culturable microbes in the mammary glands during the pathogenesis of M. bovis mastitis in the bovine host to understand a complete picture of these interactions in maintaining healthy homeostatic state or mastitis.
2025-02-04 | GSE269146 | GEO
Project description:Embryonic bovine lung (EBL) cells were infected with M. bovis for 12h and 24h, and the uninfected cells were used as the control group for 4D-DIA proteomic analysis to screen the host proteins associated with M. bovis infection. GO,KEGG analysis was performed to reveal the response mechanism of EBL cells to Mycoplasma bovis infection.
2024-11-21 | PXD058118 |
Project description:Infections with mycobacteria, including Mycobacterium tuberculosis (Mtb) and Mycobacterium bovis (M. bovis) BCG, are a leading cause of morbidity and mortality for HIV-infected persons. In contrast to HIV, SIV infection of sooty mangabeys is nonpathogenic, characterized by a lack of clinical disease including an absence of opportunistic infections. The goal of this study was to identify innate immune responses to M. bovis BCG maintained during nonpathogenic lentiviral infections, through a comparison of functional responses during pathogenic HIV or nonpathogenic SIV infections. Monocytes were evaluated for their ability to express key anti-mycobacterial cytokines TNF-α and IL-12 following a six-hour ex vivo BCG exposure. While HIV-infection was associated with a decreased percentage of IL-12-producing monocytes, nonpathogenic SIV-infection was associated with an increased percentage of monocytes producing both cytokines. Gene expression analysis of PBMC following ex vivo BCG exposure identified differential expression of NK cell-related genes and several cytokines, including IFN-γ and IL-23, between HIV-infected and control subjects. In contrast, SIV-infected and uninfected-control mangabeys exhibited no significant differences in gene expression after BCG exposure. Finally, differential gene expression patterns were identified between species, with mangabeys exhibiting lower IL-6 and higher IL-17 in response to BCG when compared to humans. Overall, this comparison of immune responses to M. bovis BCG identified unique immune signatures (involving cytokines IL-12, TNF-α, IL-23, IL-17, and IL-6) that are altered during HIV, but maintained or increased during nonpathogenic SIV infections. These unique cytokine and transcriptome signatures provide insight into the differential immune responses to Mycobacteria during pathogenic HIV-infection of humans or nonpathogenic SIV-infection of mangabeys.
2016-05-27 | GSE81926 | GEO