Ontology highlight
ABSTRACT:
PROVIDER: PRJNA214881 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1204566.fastq.gz | Fastqsanger.gz | |||
| SRR1708060_1.fastq.gz | Fastqsanger.gz | |||
| SRR1708060_2.fastq.gz | Fastqsanger.gz | |||
| SRR1708061_1.fastq.gz | Fastqsanger.gz | |||
| SRR1708061_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 68 |
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Project description:Somatic cells can be directly reprogrammed to pluripotency by exogenous expression of transcription factors, classically Oct4, Sox2, Klf4 and c-Myc. While distinct types of somatic cells can be reprogramed with varying efficiencies and by different modified reprogramming protocols, induced pluripotent stem cell (iPSC) induction remains inefficient and stochastic where a fraction of the cells converts into iPSCs. The nature of rate limiting barrier(s) preventing majority of cells to convert into iPSCs remains elusive. Here we show that neutralizing Mbd3, a core member of the Mbd3/NURD co-repressor and chromatin-remodeling complex, results in deterministic and synchronized reprogramming of multiple differentiated cell types to pluripotency. 100% of Mbd3 depleted mouse and human somatic cells convert into iPSCs after seven days of reprogramming factor induction. Our findings delineate a critical pathway blocking the reestablishment of pluripotency, and offer a novel platform for future dissection of epigenetic dynamics leading to iPSC formation at high resolution.
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