Project description:Analysis of HL60 response to low-dose vincristine at gene expression level. The hypothesis tested in the present study was that population heterogeneity has functional consequences in drug response. Since the presence of discrete efflux^High and efflux^Low subpopulations may reflect transitions between distinct stable cellular states (attractor states), we measured the transcriptomes when the cell population exhibited a stable bimodal distribution. We also measured the transcriptomes for 17 days after washing the drug (samples 5 - 9). Total RNA obtained from HL60 cells untreated (sample 3), treated for 24h (sample 4) and sorted after 24h treatment for Calcein AM efflux low (sample 1) and high (sample 2). Sample 5 corresponds to total RNA of untreated cells, sample 6 to total RNA after 24h treatment with 10nM vincristine and samples 7 to 9 to total RNA after washing the drug.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
