Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA215215 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) infected with retrovirous encoding DP1-shRNA or control-shRNA.
2014-04-10 | GSE49861 | GEO
Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) infected with retrovirous encoding DP1-shRNA or control-shRNA. TIG-3 cells were subjected to infection with retrovirus encoding DP1-shRNA or control-shRNA. Total RNA was isolated using TRIzol reagent and were analyzed using the human 3D-Gene DNA chip (Toray) that contains 25000 genes. The genome wide transcriptional
2014-04-10 | E-GEOD-49861 | biostudies-arrayexpress
Project description:Here we report the identification of genomic regions of DNA bound by Dp1 in Drosophila S2R+ cells. Dp1 is a dimerization partner of several E2F transcription factors and is needed for E2F target promoter binding. We find that Dp1 binds the promoter regions of genes important for oxidative phosphorylation. This result is important since our data demonstrates that expression of several oxidative phosphorylation genes is down-regulated in dDP mutant Drosophila 3rd instar larval eye imaginal discs. These ChIP-seq results suggest that the mechanism by which dDP regulates expression of these genes is direct. In addition, we have confirmed a number of these Dp1 bound gene promoters by conventional Chromatin Immunoprecipitation. Examination of Dp1 bound regions of genomic DNA in S2R+ cells.
2013-11-25 | E-GEOD-39393 | biostudies-arrayexpress
Project description:Effects of fzr1 knockdown on senescence-associated gene expression in human diploid fibroblasts.
| PRJNA144285 | ENA
Project description:Expression data from human fetal lung normal diploid fibroblasts
| PRJNA253353 | ENA
Project description:Here we report the identification of genomic regions of DNA bound by Dp1 in Drosophila S2R+ cells. Dp1 is a dimerization partner of several E2F transcription factors and is needed for E2F target promoter binding. We find that Dp1 binds the promoter regions of genes important for oxidative phosphorylation. This result is important since our data demonstrates that expression of several oxidative phosphorylation genes is down-regulated in dDP mutant Drosophila 3rd instar larval eye imaginal discs. These ChIP-seq results suggest that the mechanism by which dDP regulates expression of these genes is direct. In addition, we have confirmed a number of these Dp1 bound gene promoters by conventional Chromatin Immunoprecipitation.
2013-11-25 | GSE39393 | GEO
Project description:Gene expression profile of replicative senescence in normal human diploid fibroblasts.
| PRJNA215213 | ENA
Project description:Gene expression profile of p16-induced senescence in normal human diploid fibroblasts.
| PRJNA215212 | ENA
Project description:E2F is a family of master transcription regulators involved in mediating diverse cell fates, and loss of control of the pathway is regarded as a hallmark of cancer. Recent studies have highlighted the pRb-E2F axis as a regulator of a large cellular network, which in addition to its classical target genes includes RNA splicing and non-coding genes. Here, we have addressed the generality of these effects by studying the role of the other key components of the pathway, including pRb, DP and PRMT5. Like E2F1, both pRb and DP1 influence transcription and alternative RNA splicing (AS) which is additionally impacted by PRMT5 activity. A detailed proteomics analysis of the E2F1 interactome revealed SRSF2 and HNRNPC as partner proteins that assist E2F1 in mediating the effects on RNA splicing. The ability of E2F1 to influence AS activity was apparent as cells progress through the cell cycle and during the DNA damage response. Our results highlight the role of the pRb-E2F pathway in mediating cell cycle regulation of gene expression mediated by transcription and RNA splicing, and provide a mechanistic understanding for how this is likely to occur.
2026-02-09 | PXD056505 | Pride
Project description:Gene expression profile of oncogenic Ras-induced senescence in normal human diploid fibroblasts.
| PRJNA215214 | ENA