Project description:Cellular binary fate decisions require the progeny to silence genes associated with the alternative fate. The major subsets of alpha:beta T cells have been extensively studied as a model system for fate decisions. While the transcription factor RUNX3 is required for the initiation of Cd4 silencing in CD8 T cell progenitors, it is not required to maintain the silencing of Cd4 and other helper T lineage genes. The other runt domain containing protein, RUNX1, silences Cd4 in an earlier T cell progenitor, but this silencing is reversed whereas the gene silencing after RUNX3 expression is not reverse. Therefore, we hypothesized that RUNX3 and not RUNX1 recruits other factors that maintains the silencing of helper T lineage genes in CD8 T cells. To this end, we performed a proteomics screen of RUNX1 and RUNX3 to determine candidate silencing factors.

2019-11-12 | PXD007511 | Pride

Mus musculus

Project description:Runx3 function in IL-2-activated splenic CD8+ T cells.

| PRJNA216117 | ENA

E-GEOD-50122

Project description:Runx3 function in splenic NK cells (IL-2 or IL-15)

2015-07-13 | E-GEOD-50122 | ExpressionAtlas

Mus musculus

Project description:Runx3 function in splenic NK cells

| PRJNA216119 | ENA

E-GEOD-50120

Project description:Runx3 function in splenic NK cells activated in vivo by IL-15.

2015-07-13 | E-GEOD-50120 | ExpressionAtlas

Mus musculus

Project description:Runx3 function in CD4+ splenic dendritic cells

| PRJNA210763 | ENA

Genome-wide maps of Runx3 bound regions in splenic CD8+ T cells.

Project description:ChIP-seq was conducted using freshly isolated (resting) splenic WT CD8+ T cells with anti-Runx3 antibody (Ab), anti-H3K4me1 Ab and non-immune serum (NIS) as control. Two biological Runx3 and two NIS IP repeats from splenic CD8+ T cells isolated by positive selection on anti-CD8 magnetic beads, collected from 18 WT mice.

2013-11-18 | E-GEOD-50130 | biostudies-arrayexpress

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