Project description:Staphylococcus aureus is a gram-positive cocci and an important human commensal bacteria and pathogen. S. aureus infections are increasingly difficult to treat because of the emergence of highly resistant MRSA (Methicillin-resistant S. aureus) strains. Here we present a method to study differential gene expression in S. aureus using high-throughput RNA-sequencing (RNA-seq). We use RNA-seq to examine the differential gene expression in S. aureus RN4220 cells containing an exogenously expressed transcription factor and between two S. aureus strains (RN4220 and NCTC8325-4). The information provided by RNA-seq was a significant advance over previously described microarray based techniques. We investigated the sequence and gene expression differences between RN4220 and NCTC8325-4 and used the RNA-seq data to identify S. aureus promoters suitable for in vitro analysis. We used RNA-seq to describe, on a genome wide scale, genes positively and negatively regulated by a phage encoded transcription factor, gp67. RNA-seq offers the ability to study differential gene expression with single-nucleotide resolution, and is a considerable improvement over the predominant genome-wide transcriptome technologies used in S. aureus.

2013-11-07 | GSE48896 | GEO

Staphylococcus aureus subsp. aureus NCTC 8325

Project description:Transcriptome analysis of the eukaryotic-type ser/thr kinase PknB in Staphylococcus aureus

| PRJNA116575 | ENA

Effect of different Two-component systems system deletion in S. aureus NCTC8325

Project description:To analyze function of three Two-component systems SAOUHSC_00184-185, SAOUHSC_01313-1314, and SAOUHSC_01980-1981, we have employed whole genome microarray expression profiling to identify genes transcription changed in respective mutant strains. Target TCSs were replaced by ermB gene. Cells were grown to OD600=2.0, and the RNA level of wild type NCTC8325 and three TCSs mutant were compared.

2013-09-30 | E-GEOD-51197 | biostudies-arrayexpress

Staphylococcus aureus subsp. aureus NCTC 8325

Project description:The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus [Tn-seq]

| PRJNA393999 | ENA

Staphylococcus aureus subsp. aureus NCTC 8325

Project description:The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus [WGS-Seq]

| PRJNA393027 | ENA