Saccharomyces cerevisiae
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ABSTRACT:
PROVIDER: PRJNA222801 | ENA |
REPOSITORIES: ENA
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Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1∆ and slt2∆ mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid.
2013-10-18 | GSE51353 | GEO
Project description:In our previous work, we had found that Saccharomyces cerevisiae needs of the Hog1 and Slt2 proteins to growth in a low pH environment caused by sulfuric acid, one of the stress factors during the process of ethanol production. Then was performed the gene-wide expression analysis in the hog1M-bM-^HM-^F and slt2M-bM-^HM-^F mutants in order to reveal the function of the Hog1p and Slt2p MAP Kinases in the regulation of S. cerevisiae global gene expression upon stress by sulfuric acid. BY4741 strain (Reference) and their derivate mutants hog1M-bM-^HM-^F and slt2M-bM-^HM-^F were grown for 1 h in YPD medium pH 2.5 adjusted with concentrated sulfuric acid
2013-10-18 | E-GEOD-51353 | biostudies-arrayexpress
Project description:Timely signaling pathways activation allows cells to survive diverse environmental stress conditions. Mitogen-activated protein kinases (MAPKs) are a highly conserved class of signaling molecules in eukaryotes with essential functions in cellular responses to stress. In Saccharomyces cerevisiae, the role of MAPK Hog1 as a master regulator of the coordinated response to osmotic stress is well understood. However, recent findings suggest that the role of Hog1 may extend beyond canonical osmoadaptation. This study investigates the role of Hog1 in mediating transcriptional responses to acute oxidative and ethanol stress. By harnessing the natural variation present in wild strains of S. cerevisiae, we use gene knockouts, comparative transcriptomics, and survival assays to determine Hog1’s involvement in stress responses beyond osmoadaptation. Our findings demonstrate that Hog1 mediates transcriptional reprogramming for non-osmotic stress response in a strain-dependent manner. Osmospecificity of Hog1 activity was identified in the DBY8268 laboratory strain, while differential gene expression was observed in HOG1 knockouts of all wild strains tested under both oxidative and ethanol stress. Further, our data indicate that the function of Hog1 in the response to non-osmotic stress is distinct from the canonical response, with effects ranging from altered ribosomal protein expression dynamics to altered environmental stress response (ESR) activity. Differences in expression correlate with fitness defects of hog1∆ mutants. These results suggest a generalized role of the Hog1 MAPK in S. cerevisiae, consistent with an evolutionarily generalized function for this kinase, underscoring the importance of genomic diversity for elucidating stress signalling dynamics in yeast.
2026-01-29 | GSE312455 | GEO
Project description:To find how the Mck1 and Slt2 kinases regulate metabolic reprogramming in response to glucose starvation in Saccharomyces cerevisiae cells, transcriptome of wild-type cells, single and double deletion mutants of MCK1 and SLT2 grown at both glucose-replete (exponential phase) and glucose-starved (early PDS (post-diauxic shift) phase) conditions were determined.
2025-05-31 | E-MTAB-11024 | biostudies-arrayexpress
Project description:Saccharomyces cerevisiae is an excellent microorganism for industrial succinic acid production, but high succinic acid concentration will inhibit the growth of Saccharomyces cerevisiae then reduce the production of succinic acid. Through analysis the transcriptomic data of Saccharomyces cerevisiae with different genetic backgrounds under different succinic acid stress, we hope to find the response mechanism of Saccharomyces cerevisiae to succinic acid.
2022-01-09 | GSE193190 | GEO
Project description:The Yippee-like (YPEL) proteins are a conserved eukaryotic gene family implicated in proliferation, senescence, and stress adaptation. In humans, five paralogs (YPEL1–YPEL5) are widely expressed and encode proteins with high sequence similarity, but the molecular basis of their functions remains poorly defined. Functional redundancy among YPEL paralogs complicates the clarification of their individual roles. The budding yeast Saccharomyces cerevisiae has a single ortholog, MOH1, which is involved in survival and stress responses and can be functionally complemented by human YPELs. However, the cellular function of MOH1 has yet to be elucidated. Here, we investigated the function of MOH1 in S. cerevisiae. Deletion of MOH1 (moh1Δ) conferred sensitivity to sodium azide and sulfuric acid but increased resistance to hydrogen peroxide and acetic acid. Moh1 protein levels decreased upon hydrogen peroxide treatment and increased following sulfuric acid exposure, indicating stress-dependent regulation. Light and scanning electron microscopy analyses revealed that moh1Δ cells are constitutively rounder, tend to form clumps, and exhibit rough surface features, signifying altered cellular architecture. RNA profiling and FTIR spectroscopy revealed transcriptional reprogramming and metabolic remodeling in moh1Δ cells, including alterations in lipid, protein, and cell wall polysaccharide levels and composition. Intracellular ROS assays revealed that resistance to hydrogen peroxide results from reduced cellular uptake caused by altered membrane permeability, rather than from differences in mitochondrial ROS generation. Collectively, our findings identify Moh1 as a regulatory factor linking gene expression to metabolism and cellular architecture, influencing membrane permeability and conferring selective stress resistance in S. cerevisiae.
2026-06-15 | E-MTAB-16090 | biostudies-arrayexpress
Project description:Increasing evidence suggests that in disease-suppressive soils, microbial volatile compounds (mVCs) released from bacteria may inhibit the growth of plant-pathogenic fungi. However, the antifungal activities and molecular responses of fungi to different mVCs remain largely undescribed. In this study, we first evaluated the responses of pathogenic fungi to treatment with mVCs from Paenarthrobacter ureafaciens. Then, we utilized the well-characterized fungal model organism Saccharomyces cerevisiae to study the potential mechanistic effects of the mVCs. Our data showed that exposure to P. ureafaciens mVCs leads to reduced growth of several pathogenic fungi, and in yeast cells, mVC exposure prompts the accumulation of reactive oxygen species (ROS). Further experiments with S. cerevisiae deletion mutants indicated that Slt2/Mpk1 and Hog1 MAPKs play major roles in the yeast response to P. ureafaciens mVCs. Transcriptomic analysis revealed that exposure to mVCs was associated with 1030 differentially expressed genes (DEGs) in the yeast. According to GO and KEGG analyses, many of these DEGs are involved in mitochondrial dysfunction, cell integrity, mitophagy, cellular metabolism and iron uptake. Genes encoding antimicrobial proteins were also significantly altered in the yeast after exposure to mVCs. These findings suggest that oxidative damage and mitochondrial dysfunction are major contributors to the fungal toxicity of mVCs. Furthermore, our data showed that cell wall defenses, antioxidant defenses and antimicrobial defenses are induced in yeast exposed to mVCs. Thus, our findings expand upon previous research by delineating the transcriptional responses of fungal model.
2023-08-31 | GSE240052 | GEO
Project description:Wild type (BY4741) Saccharomyces cerevisiae strains and their isogenic slt2 deficient counterparts, were treated for 2 hours with sodium arsenate 100 micromolar. Control (untreated) cells were also collected. Total RNA was extracted and analyzed by microarray hybridization. The data obtained from these experiments allows to determine those genes that are regulated by Slt2 activity after arsenate treatment.
2010-07-25 | GSE17364 | GEO
Project description:We report change in the chromatin contacts upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 and CHD1) in Saccharomyces cerevisiae.
2024-02-08 | GSE227687 | GEO
Project description:We report change in the nucleosome occupancy and accessibility upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 & CHD1) in Saccharomyces cerevisiae.
2024-02-08 | GSE220880 | GEO