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Cell type-specific ribosome profiling in vivo.

ABSTRACT: Cell type-specific ribosome profiling in vivo.

PROVIDER: PRJNA223060 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1013470.fastq.gz	Fastqsanger.gz
	SRR1013480.fastq.gz	Fastqsanger.gz
	SRR1013471.fastq.gz	Fastqsanger.gz
	SRR1013481.fastq.gz	Fastqsanger.gz
	SRR1013472.fastq.gz	Fastqsanger.gz

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Cell type-specific ribosome profiling in vivo.

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Project description:Ribosome profiling has emerged as a powerful tool for genome-wide measurements of translation, but library construction requires multiple ligation steps and remains cumbersome relative to more conventional deep sequencing experiments. We report a new approach to ribosome profiling that does not require ligation. Library construction for ligation-free ribosome profiling can be completed in one day with as little as 1 ng of purified RNA footprints. We used ligation-free ribosome profiling to identify new patterns of cell type-specific translation in the brain and tested its ability to identify translational targets of mTOR signaling in the brain.

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