Project description:Our research aims to chart the circRNA expression profile and assess their impact on the lung PMN. We developed a lung PMN model and employed comprehensive RNA sequencing to analyze the differences in circRNA expression between normal and pre-metastatic lungs.Overall, our study highlights the crucial role of circRNAs in the formation of lung PMNs, supporting their potential as diagnostic or therapeutic targets for lung metastasis.

2024-09-01 | GSE274656 | GEO

Quantitative phosphoproteomic profiling of CCL5/CCR5 signaling cascade in melanoma cells

Project description:The CCL5/CCR5 axis plays a pivotal role in tumor progression and metastasis. We previously reported CCR5 promotes melanoma EMT and metastasis by upregulating TGFβ1 expression via the PI3K/AKT/GSK3β pathway. However, the full spectrum of downstream events triggered by CCR5 activation remains poorly understood. Here we employed quantitative phosphoproteomics to profile dynamic phosphorylation events in B16/F10 melanoma cells following CCL5 stimulation at three time points (5, 10 and 30 min). Temporal analysis revealed that CCL5 treatment modulated 256, 134, and 83 phosphosites at these respective time points, with a predominant bias toward upregulation. In total, 393 phosphosites across 315 phosphoproteins exhibited significant regulation. To specifically dissect CCR5-mediated phosphorylation events (distinct from other CCL5 receptors), we generated CCR5 knockout B16/F10 cells and performed comparative phosphoproteomic analysis against wild type cells at the 5-min CCL5 stimulation timepoint. This approach identified 52 phosphoproteins regulated by the CCL5/CCR5 axis, whose temporal phosphorylation dynamics were illustrated through heatmap visualization. Gene Ontology (GO) enrichment analysis revealed that CCL5/CCR5 axis participates in various biological processes, including gene expression, cell cycle, DNA repair and cytoskeleton organization. Notably, the phosphorylation of three cell cycle- associated proteins: Cep131, Khdrbs1 and Mak6 was analyzed in detail. All three proteins exhibited transient phosphorylation induction within 5-15 min of CCL5 stimulation, followed by attenuation at 15-30 min, and the phosphorylation activation was dependent on the CCR5. Overall. these findings provide a comprehensive phosphorylation resource for dissecting the molecular mechanisms underlying CCL5/CCR5-facilitated tumor progression, and provide important cues for identifying potential therapeutic targets for melanoma or other related diseases.

2026-07-14 | PXD066097 | Pride

Mus musculus

Project description:Lacrimal Gland Gene expression in Aromatase Knockout Mice and Wild Type Controls

| PRJNA97295 | ENA

Mus musculus

Project description:Differential expression between Sh2b3 knockout and wild type mice

| PRJNA273746 | ENA

Gene Expression in wild type and CCR5 knockout mice with lung metastasis

Project description:We have shown that C57BL/6J CCR5 knockout mice develop 30.4% ± 8.6% fewer B16 F10 lung nodules compared to wild type mice after the intravenous injection of 100,000 B16 F10 cells. We sought to understand this phenomenon by comparing gene expression in the lungs of these mice at 6, 24, and 48 hours after tumor injection. There were 6 groups or conditions (CCR5 - 6 hours, WT - 6 hours, CCR5 - 24 hours, WT - 24 hours, CCR5 - 48 hours, WT - 48 hour); each group had 4 to 5 mice. All of the mice were injected with 100,000 B16 F10 melanoma cells by tail vein. At the designated time, the mice were anesthetized with Avertin and their lungs were perfused with PBS. After this, the lungs were harvested and snap frozen in liquid nitrogen. They were kept at minus 80 degrees C until processing. mRNA was extracted as described below and equivalent amounts of mRNA (by weight) was pooled by group.

2013-10-25 | E-GEOD-51422 | biostudies-arrayexpress

Mus musculus

Project description:Gene Expression Profiles of Scd1 knockout mice vs wild type mice on chow diet: Liver.

| PRJNA92619 | ENA