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Transcriptional profiling of two drosophila species: Drosophila melanogaster versus Drophila sechellia

ABSTRACT: Transcriptional profiling of two drosophila species: Drosophila melanogaster versus Drophila sechellia

PROVIDER: PRJNA227140 | ENA |

REPOSITORIES: ENA

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Transcriptional profiling of two drosophila species: Drosophila melanogaster versus Drophila sechellia

Project description:Transcriptional profiling of 3 day old virgin male and female adults comparing control male Drosophila melanogaster (MDM) versus male D sechellia (MDS) and comparing control female Drosophila melanogaster (FDM) versus female D sechellia (FDS). Goal was to determine why D sechellia is tolerant to octanoïc acid, the major toxic compound of Morinda citrifolia fruit

2018-01-15 | GSE52191 | GEO

Small RNAs from four Drosophila melanogaster-subgroup species

Project description:Curration of small RNAs from four melanogaster-subgroup species (Drosophila simulans, Drosophila sechellia, Drosophila erecta, and Drosophila yakuba) for the purpose of non-coding RNA annotation and comparative genomics assessment.

2014-07-22 | GSE56244 | GEO

Cis regulatory effects on A-to-I RNA editing in Drosophila melanogaster, Drosophila sechellia and their F1 hybrids

Project description:We applied microfluidic multiplex PCR and deep sequencing (mmPCR-seq) to quantify RNA editing levels at targeted sites in Drosophila melanogaster, Drosophila sechellia and the species-specific alleles of their F1 hybrids to understand the contribution of cis and trans regulatory factors to regulating RNA editing levels.

2015-03-25 | GSE67236 | GEO

Small RNAs from four Drosophila melanogaster-subgroup species

2014-07-22 | E-GEOD-56244 | biostudies-arrayexpress

Developmental time-course study of Drosophila melanogaster, D. sechellia, D. simulans, and D. sim x D.s sec hybrids

Project description:In order to test the hypothesis that adult hybrid misexpression results from the cascading effect of earlier-expressed developmentally important improperly regulated genes, as well as address whether Von Baer’s 3rd law (suggesting that earlier stages of development should be more similar between species than later stages) holds at the level of gene expression, we conducted whole-transcriptome Drosophila melanogaster cDNA microarray-based expression profiling of males of D. melanogaster, D. sechellia, and D. simulans, at four synchronized developmental time-points (3rd instar larval [larval], early pupal, late pupal, and newly-emerged adult [adult]). D. simulans and D. sechellia shared a most recent common ancestor (MRCA) ~0.5 to 1.0 million years ago (MYA) and form a clade that shared an MRCA with D. melanogaster approximately 5.4 MYA. In addition, we also performed the same analysis on the male interspecific F1 hybrids of the D. simulans (♀) × D. sechellia (♂) cross.

2010-08-03 | GSE17535 | GEO

Genomics analysis of hexanoic acid exposure in Drosophila species

Project description:We analyze the gene expression response to hexanoic acid in Drosophila simulans, Drosophila melanogaster, and Drosophila sechellia

2021-10-04 | GSE185176 | GEO

Proteomic Analysis of Drosophila melanogaster mtx2 (Ortholog of CG8004) Mutants

Project description:Proteomic Analysis (MS/MS) of Drosophila melanogaster mtx2 (Ortholog of CG8004) Heterozygous versus Homozygous Mutants at 2 Days Post-Pupa Formation

| PXD062657 |

Drosophila sechellia

Project description:The fruit fly Drosophila sechellia is a member of the melanogaster group of the subgenus Sophophora

| PRJNA12709 | ENA

Expression divergence of chemosensory genes between Drosophila sechellia and its sibling species and its implications for host shift [Dsec JP]

Project description:Drosophila sechellia relies exclusively on the fruits of Morinda citrifolia, which are toxic to most insects, including its sibling species D. melanogaster and D. simulans. Although several odorant binding protein (Obp) genes and olfactory receptor (Or) genes were suggested to be associated with the D. sechellia host shift, a broad view of how chemosensory genes have contributed to this shift is still lacking. We therefore studied the antennal transcriptomes, the main organ responsible for detecting food resource and oviposition, of D. sechellia and its two sibling species. We wanted to know whether gene expression, particularly chemosensory genes, has diverged between D. sechellia and its two sibling species. Using a very stringent definition of differential gene expression, we found 147 genes (including 11 chemosensory genes) were up-regulated while only 81 genes (including 5 chemosensory genes) were down-regulated in D. sechellia. Interestingly, Obp50a exhibited the highest up-regulation, a ~100 fold increase, and Or85c – previously reported to be a larva-specific gene– showed ~20 fold up-regulation in D. sechellia. Furthermore, Ir84a, proposed to be associated with male courtship behavior, is significantly up-regulated in D. sechellia. We also found expression divergence in most of the receptor gene families between D. sechellia and the two sibling species. Our observations suggest that the host shift of D. sechellia is associated with expression profile divergence in all chemosensory gene families and is achieved mostly by up-regulation of chemosensory genes.

2015-10-01 | GSE67861 | GEO

Expression divergence of chemosensory genes between Drosophila sechellia and its sibling species and its implications for host shift [Dsec TW]

2015-10-01 | GSE67587 | GEO