Ontology highlight
ABSTRACT:
PROVIDER: PRJNA230447 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this experiment we sequence the whole genome DNA of 28 unique populations of the root-knot nematode Meloidogyne hapla to investigate their genetic diversity using illumina sequencing. The hapla populations came from various locations, mostly in the Netherlands and Europe. The nematodes were maintained on tomato cv. Money Maker in the greenhouse of Wageningen University. After DNA extraction, DNA was sent for sequencing. The resulting sequence was used for calling variants and genetic analysis.
2026-07-31 | E-MTAB-17107 | biostudies-arrayexpress
Project description:Plant-parasitic nematodes (PPNs) pose major agricultural threats, yet their transcriptional regulatory mechanisms remain poorly understood. Deciphering these mechanisms is critical for advancing knowledge of nematode development and host interactions, and for guiding the rational design of integrated control strategies. However, the lack of reliable synchronization of nematode development within plant hosts, together with the stringent requirements of high-throughput sequencing library preparation, hinders comprehensive transcriptomic profiling across distinct developmental stages. To overcome this limitation, high-resolution, single-nematode RNA-seq data was generated for eight developmental stages of Meloidogyne incognita (from second-stage juveniles to adults) using a low-input Smart-seq2 approach. The resulting dataset comprises 75 high-quality transcriptome libraries with at least five biological replicates per stage, enabling robust and reproducible analyses.
2025-12-30 | GSE315311 | GEO
Project description:Guava (Psidium guajava), referred to as the "tropical apple," is esteemed for its sweet flavor, nutritional density, and medicinal attributes, being rich in ascorbic acid, phenolics, carotenoids, fibers, and minerals. Despite its agricultural significance, guava cultivation faces considerable challenges from plant-parasitic nematodes (PPN), particularly root-knot nematodes (RKN; Meloidogyne spp.). In South America, Meloidogyne enterolobii causes severe root damage and economic losses to this crop. Plants fight nematodes through complex immune mechanisms involving pattern recognition receptors (PRRs) and signaling pathways, such as pattern-triggered immunity (PTI). The present research employed comparative shotgun proteomic analysis complemented by microscopic imaging and histochemical assays of roots from susceptible Psidium guajava and resistant P. guineense, inoculated or not with M. enterolobii, Plant-nematode interactions revealed intricate cellular responses such as giant cells formation, hypersensitivity reactions, and biochemical pathway adjustments in sucrose transport and antioxidant enzyme activities. Synthesis and accumulation of secondary metabolites like terpenes, alkaloids, and phenolics in inoculated and resistant plants were positively correlated to plant resilience. The 70 kDa heat shock protein (HSP70) emerged as pivotal in plant response, being upregulated during nematode infection.
2025-09-08 | PXD058189 | Pride
Project description:Genome sequencing of the plant parasitic nematode Meloidogyne enterolobii
| PRJEB36431 | ENA
Project description:Arthrobotrys flagrans, a typical nematode-trapping fungus (NTF) that produces a three-dimensional adhesive network to capture nematodes, has excellent potential for the de-velopment of biocontrol agents against both plant and animal parasitic nematodes. Proteins containing the common fungal extracellular membrane (CFEM) domain are important for the nematodes' trapping by A. flagrans. The loss of AfCFEM1 and AfCFEM3 resulted in a significant upregulation of proteins associated with fungal pathogenicity, forming a denser adhesive material on the trap surface and ultimately increasing nematode mortality. However, the disruption of AfCFEM2 led to the opposite result. Furthermore, the deletion of AfCFEM1-3 not only affected trap morphology, resulting in an increased proportion of irregular traps (i.e., trap cells not fused to the hyphae), but also led to a thinner cell wall of the traps. Besides, the compensate effects among the CFEM family and other families were demonstrated. This study revealed that the AfCFEM1-3 genes in A. flagrans participated in nematode adhesion, cell wall formation, and intercellular communication, providing new insights into the functions of AfCFEM in the process of nematode trapping by NTF.
| PXD067507 |