Ontology highlight
ABSTRACT:
PROVIDER: PRJNA230447 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Plant-parasitic nematodes (PPNs) pose major agricultural threats, yet their transcriptional regulatory mechanisms remain poorly understood. Deciphering these mechanisms is critical for advancing knowledge of nematode development and host interactions, and for guiding the rational design of integrated control strategies. However, the lack of reliable synchronization of nematode development within plant hosts, together with the stringent requirements of high-throughput sequencing library preparation, hinders comprehensive transcriptomic profiling across distinct developmental stages. To overcome this limitation, high-resolution, single-nematode RNA-seq data was generated for eight developmental stages of Meloidogyne incognita (from second-stage juveniles to adults) using a low-input Smart-seq2 approach. The resulting dataset comprises 75 high-quality transcriptome libraries with at least five biological replicates per stage, enabling robust and reproducible analyses.
2025-12-30 | GSE315311 | GEO
Project description:Guava (Psidium guajava), referred to as the "tropical apple," is esteemed for its sweet flavor, nutritional density, and medicinal attributes, being rich in ascorbic acid, phenolics, carotenoids, fibers, and minerals. Despite its agricultural significance, guava cultivation faces considerable challenges from plant-parasitic nematodes (PPN), particularly root-knot nematodes (RKN; Meloidogyne spp.). In South America, Meloidogyne enterolobii causes severe root damage and economic losses to this crop. Plants fight nematodes through complex immune mechanisms involving pattern recognition receptors (PRRs) and signaling pathways, such as pattern-triggered immunity (PTI). The present research employed comparative shotgun proteomic analysis complemented by microscopic imaging and histochemical assays of roots from susceptible Psidium guajava and resistant P. guineense, inoculated or not with M. enterolobii, Plant-nematode interactions revealed intricate cellular responses such as giant cells formation, hypersensitivity reactions, and biochemical pathway adjustments in sucrose transport and antioxidant enzyme activities. Synthesis and accumulation of secondary metabolites like terpenes, alkaloids, and phenolics in inoculated and resistant plants were positively correlated to plant resilience. The 70 kDa heat shock protein (HSP70) emerged as pivotal in plant response, being upregulated during nematode infection.
2025-09-08 | PXD058189 | Pride
Project description:Genome sequencing of the plant parasitic nematode Meloidogyne enterolobii
| PRJEB36431 | ENA
Project description:Biotrophic plant pathogens have evolved sophisticated strategies to manipulate their host. They derive all of their nutrients from living plant tissues, by making intimate contact with their host while avoiding a resistance response. Rice is one of the most important crop plants worldwide and an excellent model system for studying monocotyledonous plants. Estimates of annual yield losses due to plant-parasitic nematodes on this crop range from 10 to 25% worldwide. One of the agronomically most important nematodes attacking rice is the rice root knot nematode Meloidogyne graminicola. Attack of plant roots by sedentary plant parasitic nematodes, like the root knot nematodes (RKN; Meloidogyne spp.) involves the development of specialized feeding cells in the vascular tissue. The second stage juvenile of the RKN punctures selected vascular cells with its stylet, injects pharyngeal secretions, and this ultimately leads to the reorganisation of these cells into typical feeding structures called giant cells (GCs), from which the nematode feeds for the remainder of its sedentary life cycle (Gheysen & Mitchum, 2011). Morphological and physiological reprogramming of the initial feeding cell leads to nucleus enlargement, proliferation of mitochondria and plastids, metabolic activation, cell cycle alterations and cell wall changes (Gheysen and Mitchum, 2011). The hyperplasia and hypertrophy of the surrounding cells leads to the formation of a root gall, which is typically formed at the root tips in the case of the rice RKN M. graminicola. In comparison with other RKN, M. graminicola has a very fast life cycle, with swelling of the root tips observed as early as 1 day after infection (dai). At 3 dai, terminal hook-like galls are clearly visible (Bridge et al., 2005). After 3 moults the nematodes are mature, around 10 dai. The M. graminicola females lay their eggs inside the galls, while most other RKN deposit egg masses at the gall surface, and hatched juveniles can reinfect the same or adjacent roots. In well-drained soil at 22-29 degrees C the life cycle of M. graminicola is completed in 19 days. 2 biological replicates of nematode infected giant cells and control vascular cells were sampled at two time points: 7 and 14 dai
2013-08-07 | E-GEOD-43577 | biostudies-arrayexpress
Project description:We compared the gene expression of wild-type Col-0 and a T-DNA mutant SALK_116381C (opr2-1). We either infected or mock-infected the plants with the root knot nematode Meloidogyne incognita and measured the root transcriptome after 0, 1, 4, and 7 days post infection using RNA-seq. The aim of the experiment was to determine whether opr2-1 affected gene expression patterns induced by nematode infection.
2022-12-24 | E-MTAB-11583 | biostudies-arrayexpress