Mus musculus
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-53225
PROVIDER: PRJNA231168 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Expression data from miR-92 over-expressing R26MER/MER mouse embryonic fibroblast (MEFs)
2015-07-31 | E-GEOD-53225 | ExpressionAtlas
Project description:miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was knocked into the genomic region downstream of the constitutive Rosa26 promoter, allowing acute activation of c-Myc by 4-OHT-induced nuclear translocation. This in vitro system nicely recapitulates c-Myc-induced apoptosis, as activated MycERT2 induces strong p53-dependent apoptosis in response to serum starvation. Enforced miR-92 expression in three independent R26MER/MER MEF lines significantly enhanced Myc-induced apoptosis. We used microarrays to investigate the molecular mechanism underlying miR-92 functions.
2013-12-11 | GSE53225 | GEO
Project description:miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was knocked into the genomic region downstream of the constitutive Rosa26 promoter, allowing acute activation of c-Myc by 4-OHT-induced nuclear translocation. This in vitro system nicely recapitulates c-Myc-induced apoptosis, as activated MycERT2 induces strong p53-dependent apoptosis in response to serum starvation. Enforced miR-92 expression in three independent R26MER/MER MEF lines significantly enhanced Myc-induced apoptosis. We used microarrays to investigate the molecular mechanism underlying miR-92 functions. Three independent R26MER/MER MEF lines were infected by MSCV vector alone or by MSCV vector encoding miR-92. These MEFs were serum starved and 4-OHT treated to trigger strong Myc-induced apoptosis.
2013-12-11 | E-GEOD-53225 | biostudies-arrayexpress
Project description:RNA-seq of mouse embryonic fibroblasts(MEFs) with knock-out of SERF2 compared to control MEFs
2023-06-15 | E-MTAB-10083 | ExpressionAtlas
Project description:Transcription profiling by array of mouse NIH3T3 cells over-expressing PPARg pr EBF1 over time
2014-08-29 | E-GEOD-2192 | ExpressionAtlas
Project description:RNA-Seq of human embryonic stem cells over expressing NANOS3 and DAZL
2025-01-23 | E-MTAB-3849 | ExpressionAtlas
Project description:Expression data from embryonic mouse cervical spinal cords
2015-07-15 | E-GEOD-47953 | ExpressionAtlas
Project description:Transcription profiling by array of mouse embryonic fibroblasts expressing CBX8 or BMI1
2014-07-03 | E-MEXP-793 | ExpressionAtlas
Project description:Adult beta cells in the pancreas are the sole source of insulin in our body. Beta cell loss or increased demand for insulin, impose metabolic challenges because adult beta cells are generally quiescent and infrequently re-enter the cell division cycle. miR-17-92/106b is a family of proto-oncogene microRNAs, that regulate proliferation in normal tissues and in cancer. Here, we employ mouse genetics to demonstrate a critical role for miR-17-92/106b in glucose homeostasis and in controlling insulin secretion. Mass spectrometry analysis was performed on miR-17-92LoxP/LoxP;106-25-/- MEF lysate, without or with CRE-Adenovirus. miR-17-92LoxP/LoxP;106-25+/+ MEFs with GFP-Adenovirus served as controls. We demonstrate that miR-17-92/106b regulate the adult beta cell mitotic checkpoint and that miR-17-92/106b deficiency results in reduction in beta cell mass in-vivo. Furthermore, protein kinase A (PKA) is a new relevant molecular pathway downstream of miR-17-92/106b in control of adult beta cell division and glucose homeostasis. Therefore, contributes to the understanding of proto-oncogene miRNAs in the normal, untransformed endocrine pancreas, and illustrates new genetic means for regulation of beta cell mitosis and function by non-coding RNAs.
2019-06-17 | PXD012610 | Pride
Project description:Transcription profiling by array of human gastric cancer cell line SNU638 over-expressing miR-146a
2025-01-22 | E-MEXP-3479 | ExpressionAtlas