Ontology highlight
ABSTRACT:
PROVIDER: PRJNA231842 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Small artery remodeling depends on the genetic background in spontaneously hypertensive rats (microRNA)
| PRJNA231845 | ENA
Project description:Small artery remodeling depends on the genetic background in spontaneously hypertensive rats (mRNA)
| PRJNA231846 | ENA
Project description:We investigated morphometric structure and gene expression by microarray analysis in a small diameter artery, branch of the saphenous artery (a resistance artery), in representative models of renin-angiotensin system (RAS)-dependent and glucocorticoid hypertension, using the spontaneously hypertensive rat (SHR) and adrenocorticotropic hormone (ACTH)-induced hypertensive rat, respectively. Sixteen-week-old male Wistar-Kyoto (WKY) and age-matched spontaneously hypertensive rats (SHR) were used. Keywords: Comparison of global gene expression in resistance arteries of normotensive and genetically hypertensive rats and ACTH-treated rats.
2007-11-28 | GSE8051 | GEO
Project description:We investigated morphometric structure and gene expression by microarray analysis in a small diameter artery, branch of the saphenous artery (a resistance artery), in representative models of renin-angiotensin system (RAS)-dependent and glucocorticoid hypertension, using the spontaneously hypertensive rat (SHR) and adrenocorticotropic hormone (ACTH)-induced hypertensive rat, respectively. Sixteen-week-old male Wistar-Kyoto (WKY) and age-matched spontaneously hypertensive rats (SHR) were used. Experiment Overall Design: There were 3 experimental groups: Group 1: 16-week male Wistar-Kyoto rats; Group 2: 16-week male Wistar-Kyoto rats treated with ACTH (0.1mg/kg/day) subcutaneously, for 4 weeks prior to sampling (i.e. during weeks 12-16 of life) ; Group3: 16-week male SHR (spontaneously hypertensive) rats. There were 3 replicate hybridizations in each experimental group. Due to the low yield of total RNA obtained from the arterial sections, each replicate was composed of RNA pooled from 2-3 different rats.
2008-06-15 | E-GEOD-8051 | biostudies-arrayexpress
Project description:The goals of the study are to compare differently expressed genes in heart tissues of hypertensive rats (spontaneously Hypertension Rats, SHR) with age-matched control rats (wistar Rats, WKY), identify new targets to reverse hypertension induced cardiac remodeling and idetify the targets of Traditional Chinese Medicine QDG.
2020-01-31 | GSE144548 | GEO
Project description:Spontaneously hypertensive rats (SHR) have been used frequently as a model for human essential hypertension. However, both the SHR and its normotensive control, the Wistar Kyoto rat (WKY), consist of genetically different sublines. We tested the hypothesis that discrepant data in literature regarding the pathophysiology of vascular remodeling in hypertension result from the use of different rat sublines. Using micro-arrays, we studied miRNA and mRNA expression in resistance arteries of WKY and SHR from three different sources, at 6 weeks and 5 months of age. Both WKY and SHR showed an age-related expression pattern that involved many genes related to the extracellular matrix. In SHR, this pattern was more extensive and included a specific increase in miR132-3p, and type III deiodinase. Direct comparison of WKY to SHR also yielded differences in expression, including thrombospondin 4. Heterogeneity in gene expression among sublines was associated with differences in blood pressure, body weight, vascular remodeling, endothelial function, and vessel ultrastructure. Common features in vessels from SHR were an increase in wall thickness, wall-to-lumen ratio, and internal elastic lamina thickness. These results indicate that endothelial dysfunction, vascular stiffening, and inward remodeling of small arteries are not common features of hypertension, but are subline-dependent. Relatively minor differences in genetic background associate with different types of vascular remodeling in hypertensive rats. The clinical implication of this study is that more research into personalized treatment in hypertension is warranted.
2015-10-02 | GSE53361 | GEO
Project description:Vascular aging is directly related to several major diseases including hypertension and atherosclerosis, in which endothelial dysfunction and smooth muscle phenotype changes are crucial. However, cell types within the vessel wall and their dynamic cellular communication status have not been characterized in different arteries during hypertensive aging. To depict the interconnectedness of complex mechanism between hypertension and aging, we performed single cell RNA sequencing of aorta, femoral and mesentery artery, respectively from Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) aging 16-72 weeks. We found that aging and hypertension alone have a significant impact on the alteration of cellular composition and artery remodeling both on conductive and resistant arteries, even greater when superimposed. Consistently, smooth muscle cells (SMCs) underwent phenotypic switching from contractile towards synthetic, apoptotic and senescent SMCs with aging/hypertension. We identified three sub-clusters of Spp1high synthetic SMCs, Spp1high matrix activated fibroblasts and Spp1high scar-associated macrophage involved in hypertensive aging, highlighting that Spp1, encoding protein osteopontin (OPN), could be a potential regulator participating in hypertensive aging. Spp1high scar-associated macrophage enriched for ROS metabolic process, supramolecular fiber organization and actin filament organization. Cell-cell communication analysis also revealed SPP1-Cd44 receptor pairing was markedly aggravated on hypertensive aging condition. Importantly, the concentration of OPN in human serum significantly potentiated in hypertension patient compared with normal group. Thus, we provide a comprehensive cell atlas to systematically resolve the cellular diversity and dynamic cellular communication changes of the vessel wall during hypertensive aging, highlight the mechanisms of vascular vasodilation through cGMP-PKG signaling pathway and identified a protein marker osteopontin as a potential regulator of vascular remodeling during hypertensive aging.
2025-03-20 | GSE229178 | GEO
Project description:Spontaneously hypertensive rats (SHR) have been used frequently as a model for human essential hypertension. However, both the SHR and its normotensive control, the Wistar Kyoto rat (WKY), consist of genetically different sublines. We tested the hypothesis that discrepant data in literature regarding the pathophysiology of vascular remodeling in hypertension result from the use of different rat sublines. Using micro-arrays, we studied miRNA and mRNA expression in resistance arteries of WKY and SHR from three different sources, at 6 weeks and 5 months of age. Both WKY and SHR showed an age-related expression pattern that involved many genes related to the extracellular matrix. In SHR, this pattern was more extensive and included a specific increase in miR132-3p, and type III deiodinase. Direct comparison of WKY to SHR also yielded differences in expression, including thrombospondin 4. Heterogeneity in gene expression among sublines was associated with differences in blood pressure, body weight, vascular remodeling, endothelial function, and vessel ultrastructure. Common features in vessels from SHR were an increase in wall thickness, wall-to-lumen ratio, and internal elastic lamina thickness. These results indicate that endothelial dysfunction, vascular stiffening, and inward remodeling of small arteries are not common features of hypertension, but are subline-dependent. Relatively minor differences in genetic background associate with different types of vascular remodeling in hypertensive rats. The clinical implication of this study is that more research into personalized treatment in hypertension is warranted.
2015-10-02 | GSE53363 | GEO
Project description:We used spontaneously hypertensive rats (SHRs) as an animal model of hypertensive heart disease and treated them with allisartan orally. We continuously monitored the rats' blood pressure levels, measured their body and heart weights, and evaluated their cardiac structure and function using echocardiography. We performed proteome analysis using the tandem mass tag (TMT) technology.
2024-02-27 | PXD048882 | Pride
Project description:This study aimed at integrating metabolomics and proteomics data for a comprehensive view of the molecular targets of intervention of protein extracts from Tenebrio molitor in treating hypertension. Serum samples from spontaneously hypertensive rats and Wistar Kyoto rats were analyzed using a quantitative metabolomics and label-free proteomics approach based on liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS). Among deregulated metabolites and proteins in hypertensive rats, we found 15 metabolites and 17 proteins that were restored by supplementation with Tenebrio molitor protein extract. The combination of metabolomics and proteomics provided useful data to uncover the molecular targets of intervention and the underlying functional mechanism of Tenebrio molitor protein extract in an animal model such as spontaneously hypertensive rats. The results suggested that Tenebrio molitor supplementation could effectively treat hypertension, partially by regulating proteins and molecules mainly involved in biological pathways associated to angiotensinogen-angiotensin, Serin protease inhibitors, kallikrein–kinin, reactive oxygen scavenging, and lipid peroxidation.
2022-10-13 | PXD034456 | Pride