Project description:Bone marrow neutrophils are highly heterogeneous cells and we used sing cell RNA-sequencing to analyze the diversity of BM neutrophils of SAMP6 and SAMR1 mice femur bone.
Project description:ATAC-seq profiling of Nfat5 KO and wild type macrophages derived from bone marrow (primary cells), treated or not with Lipopolysaccharide (LPS).
Project description:We performed ATAC-seq of 7,000-50,000 ex-vivo flow-sorted cells from the granulopoietic tree in mice. For this we sorted Granulocyte-Macrophage Progenitors (GMP) from bone marrow (BM) and spleen (SP), Pre-Neutrophils (PreN) from BM and SP as well as Immature Neutrophils (imNeu) and Mature neutrophils (Neutro) from BM, SP and Blood (Bld). After sorting, cells were immediately processed using the OmniATAC protocol (Corces, Nat Methods 2014) and sequenced on a Novaseq-6000.
