Project description:To determine what signalling pathways are affected by LILRB1 in MM cells, ARP-1 MM cell lines were transfected with lentivirus to knockdown LILRB1, injected to nsg mice, sorted from the bone marrow of NSG mice and sent for RNA-seq. Total RNAs of 2 x 10^6 CTR-KD ARP-1 cells or LILRB1-KD ARP-1 cells were extracted by RNeasy Mini Kit (Qiagen). 5-10 µg RNA samples were sent to Cancer Genomics Center at The University of Texas (Houston, TX) for RNA-seq followed by data analysis. We use the RNA-seq data to determine differential expression of genes in CTR-KD ARP-1 cells and LILRB1-KD ARP-1 cells.

2024-03-07 | GSE226821 | GEO

ARP-1 wild-type and ARP-1 carfilzomib-resistant cells RNA-seq analysis

Project description:ARP-1 wild-type and ARP-1 carfilzomib-resistant cells RNA-seq analysis

| PRJNA1257777 | ENA

Establishing a molecular phenotype for Angelman Syndrome stem cell-derived neurons

Project description:mRNAseq on (1) isogenic control and Angelman Syndrome pluripotent stem cell-derived neurons or (2) antisense oligonucleotide-treated H9 hESC-derived neurons

2021-04-01 | GSE160747 | GEO

Differences in transcription in Angelman syndrome and control person iPSC-derived neurons

Project description:Angelman syndrome is caused by loss of funtional ubiquitin E3 ligase UBE3A and results in severe deley in cognitive and motor development. In neurons, UBE3A locates to the synapse and to the nucleus. Loss of nuclear UBE3A results in development of Angelman syndrome like symptoms in mice. UBE3A can function as transcriptional coactivator of steroid hormone receptors, but the entire function of UBE3A in the nucleus is still not clear. So we wanted to study differences in the transcriptome in neurons differentiated from iPSCs that were derived from patients with Angleman syndrome and normal controls.

2020-12-31 | GSE146640 | GEO

Single nuclei RNA-sequencing of frontal cortex of a pig model of Angelman syndrome

Project description:Angelman syndrome is a neurodevelopmental disorder caused by the loss of the maternal allele of the ubiquitin-protein ligase E3A (UBE3A) gene. UBE3A is imprinted with maternal-allelic expression in neurons of the central nervous syndrome (CNS) and biallelically expressed in other cell types. Consequently, in Angelman syndrome, UBE3A is absent in CNS neurons and reduced by half in other cells. It is unclear how cell type-specific gene expression in the brain is dysregulated in Angelman syndrome. Using single nuclei RNA-sequencing, we show that gene expression is dysregulated in neuronal subtypes in the frontal cortex of neonatal pigs with a UBE3A maternal deletion. A total of 3,812 unique genes were dysregulated across ten cell-type clusters, with most of the dysregulated genes (3,154 genes) in excitatory neurons.

2025-12-23 | GSE314025 | GEO

Angelman's Syndrome Natural History - ARP 5203

Project description:Angelman's Syndrome Natural History - ARP 5203

| PRJNA185024 | ENA

MethylC-Seq of Ws background wild-type (WT), ape1l-1 (Ws background), arp-1 (Col-0 background) and zdp-1 (Col-0 background) mutants

Project description:Using MethylC-Seq to provide single-base resolution of DNA methylation status in Ws background wild-type (WT), ape1l-1 (Ws background), arp-1 (Col-0 background) and zdp-1 (Col-0 background) mutants MethylC-Seq: 1 WT and 3 mutants examined, Ws background wild-type (WT), ape1l-1 (Ws background), arp-1 (Col-0 background) and zdp-1 (Col-0 background) mutants

2014-11-21 | E-GEOD-52983 | biostudies-arrayexpress

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