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Gut metagenome

ABSTRACT: Effects of prebiotic, probiotic and synbiotic on caecal microbial diversity of laying hens

PROVIDER: PRJNA235148 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1139620_1.fastq.gz	Fastqsanger.gz
	SRR1139620_2.fastq.gz	Fastqsanger.gz
	SRR1138341_1.fastq.gz	Fastqsanger.gz
	SRR1138341_2.fastq.gz	Fastqsanger.gz
	SRR1139731_1.fastq.gz	Fastqsanger.gz

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Project description:Synbiotics are a combination of probiotics and prebiotics which can alter the composition of the gastrointestinal tract evoking beneficial effects throughout the body through the production of a battery of bioactive metabolites. In this study, a synbiotic was used to reduce the behavioral and biochemical symptoms of depression and this nanostring panel was used to decipher where along the gut-brain-axis the synbiotic-derived metabolites were invoking their beneficial effects on the immune system. The synbitoic was composed of two probiotic bacteria (Lactobacillus fermentum ATCC 793 and Bifidobacteria longum ATCC 15707 and a grape -derived prebiotic composed of grape seed polyphenol extract, resveratrol and a concord grape extract. Male mice (C57BL/6) were pretreatment with either nothing (control), BDPP, probiotic or synbiotic and underwent 28 days of chronic unpredicitable stress. After 28 days, animals' behavior reflected an increase in depressive- and anxiety-like behavior, rescued specifically by the synbiotic. This nanostring multiplex analysis reveals both tissue- and treatment-specific effects on immune modulators.

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